公开(公告)号：EP3186396A1

公开(公告)日：2017-07-05

申请号：EP15835681.6

申请日：2015-08-28

申请人： Cedars-Sinai Medical Center ,  The Regents of the University of California ,  United States Government Represented by the Department of Veterans Affairs

发明人： TAJBAKHSH, Jian ,  MORTAZAVI, Fariborz

IPC分类号： C12Q1/68 ,  G01Q30/02 ,  G01N1/30 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  C12Q2600/154 ,  G01N1/30 ,  G01N33/57423 ,  G01N2440/12 ,  G01N2800/50

摘要： In certain embodiments, this application discloses methods for detecting lung cancer. The method includes characterization of cells extracted from human sputum, which is a valuable tissue surrogate and source of upper respiratory cells that become cancerous early in 5 the process of lung cancer development. The method includes the staining of extracted cells with fluorescent reporters that produce a specific pattern in the nuclei of labeled cells, which can be made visible by light microscopy. The pattern is relevant to a type of epigenetic coding of DNA known as DNA methylation, which changes in specific cells of the lung during cancer development, in comparison to normal respiratory cells.

摘要翻译： 在某些实施方案中，本申请公开了用于检测肺癌的方法。 该方法包括从人类痰中提取的细胞的特征，这是肺癌发展过程中早期发生癌变的有价值的组织替代物和上呼吸道细胞的来源。 该方法包括用荧光报道分子对提取的细胞进行染色，所述荧光报道分子在标记细胞的细胞核中产生特定模式，其可通过光学显微镜可见。 该模式与一种称为DNA甲基化的DNA的表观遗传编码有关，与正常呼吸细胞相比，其在癌症发展过程中在肺的特定细胞中发生变化。

公开(公告)号：EP4249608A2

公开(公告)日：2023-09-27

申请号：EP23191313.8

申请日：2015-08-28

申请人： Cedars-Sinai Medical Center ,  The Regents of the University of California ,  United States Government Represented by the Department of Veterans Affairs

发明人： TAJBAKHSH, Jian ,  MORTAZAVI, Fariborz

IPC分类号： C12Q1/6886

摘要： In certain embodiments, this application discloses methods for detecting lung cancer. The method includes characterization of cells extracted from human sputum, which is a valuable tissue surrogate and source of upper respiratory cells that become cancerous early in the process of lung cancer development. The method includes the staining of extracted cells with fluorescent reporters that produce a specific pattern in the nuclei of labeled cells, which can be made visible by light microscopy. The pattern is relevant to a type of epigenetic coding of DNA known as DNA methylation, which changes in specific cells of the lung during cancer development, in comparison to normal respiratory cells.

公开(公告)号：EP4249608A3

公开(公告)日：2024-02-14

申请号：EP23191313.8

申请日：2015-08-28

申请人： Cedars-Sinai Medical Center ,  The Regents of the University of California ,  United States Government Represented by the Department of Veterans Affairs

发明人： TAJBAKHSH, Jian ,  MORTAZAVI, Fariborz

IPC分类号： C12Q1/6841 ,  G01Q30/02 ,  G01N1/30 ,  G01N33/574 ,  C12Q1/6886

摘要： In certain embodiments, this application discloses methods for detecting lung cancer. The method includes characterization of cells extracted from human sputum, which is a valuable tissue surrogate and source of upper respiratory cells that become cancerous early in the process of lung cancer development. The method includes the staining of extracted cells with fluorescent reporters that produce a specific pattern in the nuclei of labeled cells, which can be made visible by light microscopy. The pattern is relevant to a type of epigenetic coding of DNA known as DNA methylation, which changes in specific cells of the lung during cancer development, in comparison to normal respiratory cells.