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    USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS
    1.
    发明公开
    USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS 审中-公开
    VERWENDUNG EINER UNTRANSLATIERTEN MAISREGION ZUR TRANSGENEXPRESSION BEI PFLANZEN

    公开(公告)号：EP2885412A2

    公开(公告)日：2015-06-24

    申请号：EP13752757.8

    申请日：2013-08-08

    申请人： Dow AgroSciences LLC ,  Kumar, Sandeep ,  Gupta, Manju ,  Alabed, Diaa

    发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  ALABED, Diaa

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216 ,  C12N15/8218

    摘要： Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

    摘要翻译： 提供了用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码遍在蛋白质的基因的3'非翻译区并且被工程化成位于载体的所选编码区的相应部分。 可以通过常规或基因靶向程序将载体构建体引入植物和/或植物组织中，导致选择的编码区的表达增强。 在一些实施方案中，所选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

    NOVEL MAIZE UBIQUITIN PROMOTERS
    2.
    发明公开
    NOVEL MAIZE UBIQUITIN PROMOTERS 审中-公开
    新玉米泛素促进剂

    公开(公告)号：EP3090056A1

    公开(公告)日：2016-11-09

    申请号：EP14877214.8

    申请日：2014-12-31

    申请人： Dow AgroSciences LLC

    发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  WRIGHT, Terry R. ,  JAYNE, Susan M. ,  SMITH, Doug A. ,  ALABED, Diaa

    IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/8216

    摘要： The
    Zea mays c.v . B73 Ubiquitin-1 (
    Z. mays c.v . B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same
    Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different
    Zea species, Z .
    luxurians v1.

    摘要翻译： 玉蜀黍c.v. B73泛素-1（Z.mays c.v.B73 Ubi-1）启动子在植物中驱动高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可能导致基因沉默，从而使得转基因产物的有效性降低。 提供了使用来自不同玉蜀黍物种的Ubi-1启动子的基因调节元件在植物细胞和/或植物组织中表达转基因的基因调控启动子元件，构建体和方法，Z.luuxurians v1。

    NOVEL MAIZE UBIQUITIN PROMOTERS
    3.
    发明公开
    NOVEL MAIZE UBIQUITIN PROMOTERS 审中-公开
    新玉米泛素促进剂

    公开(公告)号：EP3090055A1

    公开(公告)日：2016-11-09

    申请号：EP14876610.8

    申请日：2014-12-31

    申请人： Dow AgroSciences LLC

    发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  WRIGHT, Terry R. ,  JAYNE, Susan M. ,  SMITH, Doug A. ,  ALABED, Diaa

    IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/8216 ,  C12N15/10

    摘要： The
    Zea mays c.v. B73 Ubiquitin-1 (Z.
    mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z.
    mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different
    Zea species , Z. luxurians v2.

    摘要翻译： 玉蜀黍c.v. B73泛素-1（Z.mays c.v.B73 Ubi-1）启动子在植物中驱动高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可能导致基因沉默，从而使得转基因产物的有效性降低。 提供了基因调控启动子元件，构建体以及用于使用来自不同玉蜀黍物种Ubi-1启动子的基因调节元件在植物细胞和/或植物组织中表达转基因的方法，所述玉米Z.luxurians v2。

    METHOD AND CONSTRUCT FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER
    5.
    发明公开
    METHOD AND CONSTRUCT FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER 审中-公开
    方法和构建合成的双向ScBV启动植物基因启动子

    公开(公告)号：EP2797407A1

    公开(公告)日：2014-11-05

    申请号：EP12862691.8

    申请日：2012-11-12

    申请人： Dow AgroSciences LLC

    发明人： KUMAR, Sandeep ,  ALABED, Diaa ,  BENNETT, Sara ,  GUPTA, Manju ,  JAYNE, Susan ,  WRIGHT, Terry

    IPC分类号： A01H5/00 ,  C12N15/11 ,  C12N15/63 ,  C12N15/82

    CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

    摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

    NOVEL MAIZE UBIQUITIN PROMOTERS
    6.
    发明公开
    NOVEL MAIZE UBIQUITIN PROMOTERS 审中-公开
    新玉米泛素促进剂

    公开(公告)号：EP3090045A1

    公开(公告)日：2016-11-09

    申请号：EP14875980.6

    申请日：2014-12-31

    申请人： Dow AgroSciences LLC

    发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  WRIGHT, Terry, R. ,  JAYNE, Susan, M. ,  SMITH, Doug, A. ,  ALABED, Diaa

    IPC分类号： C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/04 ,  C12N5/10 ,  C12N15/82 ,  C12N15/87

    CPC分类号： C12N15/8216 ,  C12N15/10

    摘要： The
    Zea mays c.v. B73 Ubiquitin-1 (
    Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same
    Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different
    Z. mays genotype,
    Z. mays c.v. B104.

    摘要翻译： 玉蜀黍c.v. B73泛素-1（Z.mays c.v.B73 Ubi-1）启动子在植物中驱动高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可能导致基因沉默，从而使得转基因产物的有效性降低。 提供了基因调控启动子元件，构建体以及使用来自不同基因型Z.mays c.v.的Ubi-1启动子的基因调控元件在植物细胞和/或植物组织中表达转基因的方法。 B104。

    CONSTRUCT AND METHOD FOR SYNTHETIC BIDIRECTIONAL PLANT PROMOTER UBI1
    8.
    发明公开
    CONSTRUCT AND METHOD FOR SYNTHETIC BIDIRECTIONAL PLANT PROMOTER UBI1 审中-公开
    建造及方法合成的双向启动子植物UBI1

    公开(公告)号：EP2797406A1

    公开(公告)日：2014-11-05

    申请号：EP12861436.9

    申请日：2012-11-12

    申请人： Dow AgroSciences LLC

    发明人： KUMAR, Sandeep ,  ALABED, Diaa ,  WRIGHT, Terry ,  GUPTA, Manju

    IPC分类号： A01H5/00 ,  C12N15/11 ,  C12N15/82 ,  C12N15/29

    摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.