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    PROCESSES FOR PRODUCING INDUSTRIAL PRODUCTS FROM PLANT LIPIDS
    6.
    发明公开
    PROCESSES FOR PRODUCING INDUSTRIAL PRODUCTS FROM PLANT LIPIDS 审中-公开

    公开(公告)号：EP4303288A3

    公开(公告)日：2024-03-06

    申请号：EP23206971.6

    申请日：2015-07-07

    申请人： Nuseed Global Innovation Ltd

    发明人： VANHERCKE, Thomas ,  PETRIE, James, Robertson ,  EL TAHCHY, Anna ,  SINGH, Surinder, Pal ,  REYNOLDS, Kyle ,  LIU, Qing ,  LEITA, Benjamin, Aldo

    IPC分类号： C11B1/10 ,  A01H1/00 ,  C11C3/00 ,  C11C3/12 ,  C11B3/00 ,  C11B3/02 ,  C11C3/10

    摘要： The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and/or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and/or the total non-polar lipid content and/or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

    SEMI-RATIONAL GENOME EVOLUTION ENGINEERING METHOD FOR PLANTS
    8.
    发明公开
    SEMI-RATIONAL GENOME EVOLUTION ENGINEERING METHOD FOR PLANTS 审中-公开

    公开(公告)号：EP4299738A1

    公开(公告)日：2024-01-03

    申请号：EP22759830.7

    申请日：2022-02-25

    申请人： National University Corporation Kobe University

    发明人： NISHIDA, Keiji ,  SHIMATANI, Zenpei ,  FUJIKURA, Ushio

    IPC分类号： C12N15/09 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C07K19/00 ,  C12N5/10 ,  C12N9/16 ,  C12N9/24

    摘要： The present disclosure provides a method for site-specific modification of DNA molecules that can be isolated as individual mutant clones.
    According to the present disclosure, a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced is provided.