摘要:
The invention relates to a method for acquiring signals in laser scanning microscopy, including the steps of: moving a focused optical excitation beam relative to an object to be measured so that the focus point of the beam follows a predetermined path in the space of said object; and acquiring optical measurement signals along said path according to at least one acquisition parameter; characterised in that the path of the excitation beam is determined so as to substantially minimise the variations of the optical properties of at least one portion of the environments crossed by said excitation beam between consecutive acquisitions, and in that at least one acquisition parameter among said acquisition parameters is modulated during the movement of the excitation beam. The invention also relates to a device for implementing said method.