公开(公告)号：EP2993228A3

公开(公告)日：2016-04-06

申请号：EP15182914.0

申请日：2015-08-28

申请人： Evonik Degussa GmbH

发明人： Evans, Ronald J. ,  Hogsett, David A. ,  Liao, Hans H. ,  Poor, Catherine B. ,  Prather, Brittany L. ,  Ribble, Wendy K. ,  Spindler, Eileen C. ,  Warnecke Lipscomb, Tanya ,  Grammann, Katrin ,  Wolter, Jan ,  Reinecke, Liv ,  Schaffer, Steffen

IPC分类号： C12N9/10 ,  C12P7/64 ,  C12N1/21

CPC分类号： C12P7/6436 ,  C12N9/1029 ,  C12Y203/01041 ,  C12Y203/01075 ,  H05K999/99

摘要： The present invention relates to a microbial cell for producing at least one fatty acid ester, wherein the cell is genetically modified to comprise
- at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by means of increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid comprises at least 5 carbon atoms; and
- a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalysing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

公开(公告)号：EP2993228A2

公开(公告)日：2016-03-09

申请号：EP15182914.0

申请日：2015-08-28

申请人： Evonik Degussa GmbH

发明人： Evans, Ronald J. ,  Hogsett, David A. ,  Liao, Hans H. ,  Poor, Catherine B. ,  Prather, Brittany L. ,  Ribble, Wendy K. ,  Spindler, Eileen C. ,  Warnecke Lipscomb, Tanya ,  Grammann, Katrin ,  Wolter, Jan ,  Reinecke, Liv ,  Schaffer, Steffen

IPC分类号： C12N9/10 ,  C12P7/64 ,  C12N1/21

CPC分类号： C12P7/6436 ,  C12N9/1029 ,  C12Y203/01041 ,  C12Y203/01075 ,  H05K999/99

摘要： The present invention relates to a microbial cell for producing at least one fatty acid ester, wherein the cell is genetically modified to comprise
- at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by means of increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid comprises at least 5 carbon atoms; and
- a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalysing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

摘要翻译： 本发明涉及用于产生至少一种脂肪酸酯的微生物细胞，其中所述细胞经遗传修饰以包含 - 至少一种第一遗传突变，其使细胞能够产生至少一种脂肪酸和/或酰基辅酶A（ CoA）依赖于丙二酰辅酶A依赖性和丙二酰辅酶A依赖性脂肪酰辅酶A代谢途径的野生型细胞中的酶活性增加，其中所述脂肪酸包含至少5个碳原子; 和 - 相对于野生型细胞和蜡酯合酶增加细胞中至少一种蜡酯合酶的活性的第二基因突变与SEQ ID NO：1-8的多肽具有至少50％的序列同一性 或其组合的任何多肽的功能片段或用于催化脂肪酸和/或其酰基辅酶A向脂肪酸酯的转化的多肽的功能片段。