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公开(公告)号:EP2993228A3
公开(公告)日:2016-04-06
申请号:EP15182914.0
申请日:2015-08-28
申请人: Evonik Degussa GmbH
发明人: Evans, Ronald J. , Hogsett, David A. , Liao, Hans H. , Poor, Catherine B. , Prather, Brittany L. , Ribble, Wendy K. , Spindler, Eileen C. , Warnecke Lipscomb, Tanya , Grammann, Katrin , Wolter, Jan , Reinecke, Liv , Schaffer, Steffen
CPC分类号: C12P7/6436 , C12N9/1029 , C12Y203/01041 , C12Y203/01075 , H05K999/99
摘要: The present invention relates to a microbial cell for producing at least one fatty acid ester, wherein the cell is genetically modified to comprise
- at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by means of increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid comprises at least 5 carbon atoms; and
- a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalysing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.-
公开(公告)号:EP2322598A3
公开(公告)日:2011-07-13
申请号:EP10188993.9
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要: Gegenstand der Erfindung sind gentechnisch modifizierte Candida tropicalis Zellen, deren Verwendung sowie ein Verfahren zur Herstellung von ω-Hydroxy-Carbonsäuren und ω-Hydroxy-Carbonsäureestern.
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公开(公告)号:EP2602313B1
公开(公告)日:2017-04-12
申请号:EP13158312.2
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
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公开(公告)号:EP3020819A1
公开(公告)日:2016-05-18
申请号:EP14193474.5
申请日:2014-11-17
申请人: Evonik Degussa GmbH
发明人: Haas, Thomas , Pötter, Markus , Schaffer, Steffen
IPC分类号: C12P7/64
CPC分类号: C12P7/6409 , Y02P20/59
摘要: The present invention relates to at least one method of producing at least one fatty acid and/or derivative thereof from a gas comprising H 2 , CO 2 and O 2 the method comprising the steps of:
(c) providing a genetically modified hydrogen oxidising bacterium in an aqueous medium; and
(d) contacting the aqueous medium with a gas comprising H 2 , CO 2 and O 2 in a weight ratio of 20 to 70: 10 to 45: 5 to 35;
wherein the fatty acid comprises at least 5 carbon atoms and
wherein the hydrogen oxidising bacterium is genetically modified relative to the wild type bacterium to increase the expression of enzyme E 1 that is capable of catalysing the conversion of acetyl CoA to acyl ACP via malonyl coA and to increase the expression of enzyme E 2 that is capable of catalysing the conversion of Acyl ACP to the fatty acid.摘要翻译: 本发明涉及至少一种由包含H 2,CO 2和O 2的气体生产至少一种脂肪酸和/或其衍生物的方法,所述方法包括以下步骤:(c)提供经遗传修饰的氢氧化细菌 在水介质中; 和(d)使水性介质与包含H 2,CO 2和O 2的气体以20至70:10至45:5至35的重量比接触; 其中所述脂肪酸包含至少5个碳原子,并且其中所述氢氧化细菌相对于野生型细菌被遗传修饰以增加能够通过丙二酰辅酶A催化乙酰辅酶A转化为酰基ACP的酶E 1的表达,以及 以增加能够催化酰基ACP转化为脂肪酸的酶E 2的表达。
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公开(公告)号:EP2602312B1
公开(公告)日:2017-04-12
申请号:EP13158308.0
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
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公开(公告)号:EP2602312A1
公开(公告)日:2013-06-12
申请号:EP13158308.0
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要: Gegenstand der Erfindung sind gentechnisch modifizierte Candida tropicalis Zellen, deren Verwendung sowie ein Verfahren zur Herstellung von ω-Hydroxy-Carbonsäuren und ω-Hydroxy-Carbonsäureestern.
摘要翻译: 本发明涉及基因修饰的热带假丝酵母(Candida tropicalis)细胞,它们的用途以及制备ω-羟基羧酸和ω-羟基羧酸酯的方法。
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公开(公告)号:EP2377944A1
公开(公告)日:2011-10-19
申请号:EP11173560.1
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要: Gegenstand der Erfindung sind gentechnisch modifizierte Candida tropicalis Zellen, deren Verwendung sowie ein Verfahren zur Herstellung von ω-Hydroxy-Carbonsäuren und ω-Hydroxy-Carbonsäureestern.
摘要翻译: 念珠菌热带细胞是新的,其中细胞(与其野生型相比)表现出至少一种酶的活性降低,其由不含内含子的核酸序列编码,包括SEQ ID No.1,3,5,7,9 ,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59 ,61,63,65或67或与上述序列至少80%相同的序列,其中上述序列未定义。 包括以下独立权利要求:(1)上述细胞的制备; 和(2)制备ω-羟基羧酸或其酯,其中羧酸的链长为6-24℃,酯的醇组分的链长为1-4℃(优选为12-羟基十二烷酸或其甲酯) 包括(a)使上述细胞与含有羧酸或其酯的培养基接触,(b)在使羧酸或其酯形成相应的ω-羟基羧酸或其酯的条件下培养细胞,和(c)任选地 分离形成的ω-羟基羧酸或其酯。
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公开(公告)号:EP2322598A2
公开(公告)日:2011-05-18
申请号:EP10188993.9
申请日:2010-10-27
申请人: Evonik Degussa GmbH
CPC分类号: C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要: Gegenstand der Erfindung sind gentechnisch modifizierte Candida tropicalis Zellen, deren Verwendung sowie ein Verfahren zur Herstellung von ω-Hydroxy-Carbonsäuren und ω-Hydroxy-Carbonsäureestern.
摘要翻译: 本发明涉及遗传修饰的热带假丝酵母的细胞,它们的用途和用于E-羟基羧酸和e羟基 - 羧酸酯的制备方法。
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