公开(公告)号：EP1896857A1

公开(公告)日：2008-03-12

申请号：EP06780818.8

申请日：2006-06-29

申请人： FUJIFILM Corporation

发明人： ABE, Yoshihiko, c/o Fuji Film Corporation ,  KAGEYAMA, Shigeki, c/o Fuji Film Corporation ,  HIRAI, Hiroyuki, c/o Fuji Film Corporation ,  KOJIMA, Masayoshi, c/o Fuji Film Corporation

IPC分类号： G01N33/92 ,  G01N33/48

CPC分类号： G01N33/5434 ,  G01N33/92 ,  G01N2446/80

摘要： An object to be achieved by the present invention is to provide a method for determining the amount of a component contained in a specific lipoprotein fraction in a biological sample using an automatic analyzer, without the requirement of a step of fractionating a sample by centrifugation. The present invention provides a method for separating a target component in a biological sample, which comprises the steps of: (1) causing a biological sample to come into contact with independently dispersed magnetic nanoparticles having a particle size of 50 nm or less, which have anionic functional groups on their surfaces, so as to form an agglutinate of the magnetic nanoparticles and biomolecules capable of interacting with the magnetic nanoparticles; and (2) collecting the agglutinate by an external magnetic field.

摘要翻译： 本发明要实现的目的是提供一种使用自动分析仪测定生物样品中特定脂蛋白级分中所含成分的量的方法，而不需要通过离心分离样品的步骤。 本发明提供了一种用于分离生物样品中的目标组分的方法，其包括以下步骤：（1）使生物样品与独立分散的具有50nm或更小粒径的磁性纳米粒子接触，所述磁性纳米粒子具有50nm或更小的粒径 阴离子官能团，从而形成磁性纳米颗粒和能够与磁性纳米颗粒相互作用的生物分子的凝集物; 和（2）通过外部磁场收集凝集物。