公开(公告)号：EP2956474B1

公开(公告)日：2018-08-22

申请号：EP14701200.9

申请日：2014-01-23

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： VILCINSKAS, Andreas ,  PÖPPEL, Anne-Kathrin ,  WIESNER, Jochen

IPC分类号： C07K14/435 ,  C12N15/82

CPC分类号： C12N15/8282 ,  A01N37/46 ,  A01N63/02 ,  C07K14/00 ,  C07K14/43577

摘要： The present invention discloses polypeptides comprising an amino acid sequence being identical with at least 12 contiguous amino acid residues of SEQ ID No 2. The polypeptides according to the invention are effective against fungi, especially against fungi causing plant diseases, and against fungi colonizing agricultural products. The invention further discloses processes for preparing such polypeptides, and nucleic acids coding for such polypeptides. In addition, the invention relates to processes and preparations for treating plants using the polypeptides according to the invention, and to the use of the nucleic acids according to the invention for producing crops that are protected against damage from fungi.

公开(公告)号：EP3614838A1

公开(公告)日：2020-03-04

申请号：EP18770258.4

申请日：2018-03-20

申请人： Dow AgroSciences LLC ,  Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： NARVA, Kenneth E. ,  GENG, Chaoxian ,  FREY, Meghan ,  GANDRA, Premchand ,  VILCINSKAS, Andreas ,  YOUNG, Catherine D. ,  BALACHANDRAN, Abhilash ,  KNORR, Eileen ,  FISCHER, Rainer

IPC分类号： A01N25/08 ,  C12N1/21 ,  C12N1/15

公开(公告)号：EP3322809A1

公开(公告)日：2018-05-23

申请号：EP16825259.1

申请日：2016-07-15

申请人： Dow Agrosciences LLC ,  Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.

发明人： NARVA, Kenneth E. ,  WORDEN, Sarah E. ,  FREY, Meghan ,  RANGASAMY, Murugesan ,  GANDRA, Premchand ,  LO, Wendy ,  FISHILEVICH, Elane ,  VILCINSKAS, Andreas ,  KNORR, Eileen

IPC分类号： C12N15/82 ,  C12N15/113 ,  C07K14/325 ,  A01N57/16 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C07K14/43563 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  Y02A40/162

摘要： This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：EP3268479A1

公开(公告)日：2018-01-17

申请号：EP16765545.5

申请日：2016-03-14

申请人： Dow Agrosciences LLC ,  Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： NARVA, Kenneth E. ,  WORDEN, Sarah E. ,  FREY, Meghan ,  RANGASAMY, Murugesan ,  GANDRA, Premchand ,  VEERAMANI, Balaji ,  LO, Wendy ,  VILCINSKAS, Andreas ,  KNORR, Eileen ,  FISHILEVICH, Elane

IPC分类号： C12N15/82 ,  C12N15/113 ,  C07K14/325 ,  A01H5/00

CPC分类号： C12N15/8286 ,  A01N57/16 ,  A01N63/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/531 ,  Y02A40/162

摘要： This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：EP3140406A1

公开(公告)日：2017-03-15

申请号：EP15789197.9

申请日：2015-05-06

申请人： Dow AgroSciences LLC ,  Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.

发明人： NARVA, Kenneth E. ,  WORDEN, Sarah E. ,  FREY, Meghan L.F. ,  RANGASAMY, Murugesan ,  ARORA, Kanika ,  VEERAMANI, Balaji ,  GANDRA, Premchand ,  VILCINSKAS, Andreas ,  KNORR, Eileen

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N5/04 ,  A01H5/00

CPC分类号： C12N15/113 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

摘要： This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

摘要翻译： 本公开涉及核酸分子及其通过RNA干扰介导的鞘内鳞翅目害虫中靶编码和转录非编码序列的抑制来控制鞘翅目害虫的方法。 本公开还涉及用于制备表达可用于控制鞘翅目害虫的核酸分子的转基因植物以及由此获得的植物细胞和植物的方法。

公开(公告)号：EP3411487A1

公开(公告)日：2018-12-12

申请号：EP17748009.2

申请日：2017-01-31

申请人： Dow Agrosciences LLC ,  Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： NARVA, Kenneth E. ,  WORDEN, Sarah E. ,  FREY, Meghan ,  RANGASAMY, Murugesan ,  GANDRA, Premchand ,  LO, Wendy ,  FISHILEVICH, Elane ,  FISCHER, Rainer ,  VILCINSKAS, Andreas ,  KNORR, Eileen

IPC分类号： C12N15/82 ,  C12N15/113 ,  C07K14/325 ,  A01N63/02

CPC分类号： C12N15/8286 ,  A01H4/008 ,  A01N37/46 ,  A01N57/16 ,  C07K14/325 ,  C07K14/43563 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8261 ,  C12N2310/14 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  Y02A40/162

摘要： This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：EP3024481B1

公开(公告)日：2018-07-11

申请号：EP14744338.6

申请日：2014-07-28

申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.

发明人： VILCINSKAS, Andreas ,  FISCHER, Rainer , 

IPC分类号： A61K38/57 ,  C07K14/81

CPC分类号： C07K14/8146 ,  A61K38/00 ,  C07K14/43563 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/40 ,  C12Q1/37 ,  G01N2333/96419

摘要： A polypeptide having at least 70% homology, in particular 80%, 90% or 95% homology to the polypeptide of SEQ ID NO:2 representing the wild-type of the protein insect metalloproteinase inhibitor IMPIα and having at least one mutation at position 35, 36 and/or 39 of the amino acid sequence of IMPIα and the polypeptide having an IC50 value to thermolysine of less than the IC50 value of IMPIα wherein the nonpolar amino acid isoleucine at position 35 of IMPIα is replaced either by a nonpolar amino acid selected from the group consisting of leucine, methionine and phenylalanine or by polar amino acid selected from the group consisting of cysteine, asparagine, glutamine, histidine, lysine and arginine; and/or the nonpolar amino acid isoleucine at position 36 of IMPIα is replaced either by a nonpolar amino acid selected from the group consisting of valine, phenylalanine and tryptophan or by polar amino acid selected from the group consisting of tyrosine, serine, threonine, asparagine, glutamine, histidine, lysine and arginine; and/or the polar amino acid position 39 of IMPIα is replaced either by the nonpolar amino acid valine or by the polar amino acids histidine or lysine.

公开(公告)号：EP3322810A1

公开(公告)日：2018-05-23

申请号：EP16825263.3

申请日：2016-07-15

申请人： Dow Agrosciences LLC ,  Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.

发明人： NARVA, Kenneth E. ,  WORDEN, Sarah E. ,  FREY, Meghan ,  RANGASAMY, Murugesan ,  GANDRA, Premchand ,  LO, Wendy ,  FISHILEVICH, Elane ,  VILCINSKAS, Andreas ,  KNORR, Eileen

IPC分类号： C12N15/82 ,  C12N15/113 ,  C07K14/325 ,  A01N57/16 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N63/00 ,  C07K14/43563 ,  C12N15/8218 ,  Y02A40/162 ,  A01N25/006

摘要： This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

公开(公告)号：EP3024481A1

公开(公告)日：2016-06-01

申请号：EP14744338.6

申请日：2014-07-28

申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.

发明人： VILCINSKAS, Andreas ,  SALZIG, Mark ,  FISCHER, Rainer

IPC分类号： A61K38/57 ,  C07K14/81

CPC分类号： C07K14/8146 ,  A61K38/00 ,  C07K14/43563 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/40 ,  C12Q1/37 ,  G01N2333/96419

摘要： A polypeptide having at least 70% homology, in particular 80%, 90% or 95% homology to the polypeptide of SEQ ID NO:2 representing the wild-type of the protein insect metalloproteinase inhibitor IMPIα and having at least one mutation at position 35, 36 and/or 39 of the amino acid sequence of IMPIα and the polypeptide having an IC50 value to thermolysine of less than the IC50 value of IMPIα wherein the nonpolar amino acid isoleucine at position 35 of IMPIα is replaced either by a nonpolar amino acid selected from the group consisting of leucine, methionine and phenylalanine or by polar amino acid selected from the group consisting of cysteine, asparagine, glutamine, histidine, lysine and arginine; and/or the nonpolar amino acid isoleucine at position 36 of IMPIα is replaced either by a nonpolar amino acid selected from the group consisting of valine, phenylalanine and tryptophan or by polar amino acid selected from the group consisting of tyrosine, serine, threonine, asparagine, glutamine, histidine, lysine and arginine; and/or the polar amino acid position 39 of IMPIα is replaced either by the nonpolar amino acid valine or by the polar amino acids histidine or lysine.

公开(公告)号：EP3625348A1

公开(公告)日：2020-03-25

申请号：EP18801372.6

申请日：2018-05-18

申请人： Dow AgroSciences LLC ,  Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： NARVA, Kenneth E. ,  GENG, Chaoxian ,  FREY, Meghan ,  GANDRA, Premchand ,  VILCINSKAS, Andreas ,  YOUNG, Catherine D. ,  BALACHANDRAN, Abhilash ,  KNORR, Eileen ,  FISCHER, Rainer

IPC分类号： C12N15/82 ,  A61K31/713