公开(公告)号：EP2365337A1

公开(公告)日：2011-09-14

申请号：EP11157638.5

申请日：2011-03-10

申请人： Fujifilm Corporation ,  National University Corporation Hokkaido University

发明人： Ogikubo, Shinya ,  Adachi, Takashi ,  Ohta, Nobuhiro ,  Nakabayashi, Takakazu

IPC分类号： G01N33/84

CPC分类号： G01N33/84 ,  A61B1/043 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/6852 ,  G01N21/6408

摘要： The pH of a substance to be measured can be measured without being influenced by the intensity of excitation light (L1), the concentration of fluorescent material and surrounding environments. In a pH measurement method, pulsed excitation light (L1) including a wavelength that can excite a predetermined fluorescent material (P) contained in living matter is generated. The fluorescent material acts as coenzyme in oxidation/reduction reaction in vivo. Further, the intensity of the pulsed excitation light (L1) does not damage a tissue nor a cell in the living matter, and does not substantially change the pH of the living matter. Further, a predetermined position in the living matter is illuminated with the pulsed excitation light (L1), and light including fluorescence emitted from the fluorescent material excited by illumination with the pulsed excitation light (L1) is received. The lifetime (Tf) of the fluorescence is calculated by time-resolving the intensity of the received fluorescence, and the pH of the living matter is measured based on the lifetime (Tf).

摘要翻译： 可以在不受激发光（L1）的强度，荧光材料的浓度和周围环境的影响的情况下测量待测物质的pH。 在pH测定方法中，生成包含活性物质中含有的能够激发规定的荧光体（P）的波长的脉冲激发光（L1）。 荧光材料在体内作为氧化/还原反应中的辅酶。 此外，脉冲激发光（L1）的强度不会损伤生物体中的组织和细胞，并且基本上不改变生物体的pH。 此外，利用脉冲激发光（L1）照射生物体中的预定位置，并且接收包含通过用脉冲激发光（L1）照射而激发的荧光体发出的荧光的光。 通过时间分辨接收到的荧光的强度来计算荧光的寿命（Tf），并且基于寿命（Tf）测量生物体的pH。

公开(公告)号：EP2365338A1

公开(公告)日：2011-09-14

申请号：EP11157639.3

申请日：2011-03-10

申请人： Fujifilm Corporation ,  National University Corporation Hokkaido University

发明人： Adachi, Takashi ,  Ogikubo, Shinya ,  Ohta, Nobuhiro ,  Nakabayashi, Takakazu

IPC分类号： G01N33/84

CPC分类号： G01N33/84 ,  A61B5/0068 ,  A61B5/0071 ,  G01N21/6408 ,  G01N21/6486

摘要： It is possible to highly accurately measure the oxygen concentration of a substance to be measured without being influenced by the intensity of excitation light (L1), the concentration of fluorescent material, and the surrounding environment. Pulsed excitation light (L1) including a wavelength that can excite a fluorescent material contained in living matter is generated. The fluorescence lifetime (Tf) of the fluorescent material is longer than or equal to 4.8 nanoseconds. A predetermined position in the living matter is illuminated with the pulsed excitation light (L1). Further, light including fluorescence (Lf) emitted from the fluorescent material excited by illumination with the pulsed excitation light (L1) is received. The lifetime (Tf) of the fluorescence (Lf) included in the received light is calculated by time-resolving the intensity of the fluorescence (Lf) . Further, the oxygen concentration of the living matter is measured based on the lifetime (Tf)

摘要翻译： 因此能够高度准确地设定测量物质中的氧浓度而不受激发光的强度（L1）荧光材料，浓度，和周围环境的影响进行测量。 脉冲激发光（L1），其包括波长确实可以激发包含在生命物质产生的荧光材料。 荧光材料的荧光寿命（TF）是长于或等于4.8毫微秒。 在生命物质的预定位置与所述脉冲的激发光（L1）照射。 此外光包括来自通过照明用脉冲的激发光（L1）激发的荧光材料发出的荧光（LF）被接收。 的荧光的寿命（TF）（LF）包括在接收到的光通过时间分辨荧光（LF）的强度来计算。 此外，的生命物质中的氧浓度是基于寿命测定（TF）

公开(公告)号：EP2365336A1

公开(公告)日：2011-09-14

申请号：EP11157637.7

申请日：2011-03-10

申请人： Fujifilm Corporation ,  National University Corporation Hokkaido University

发明人： Adachi, Takashi ,  Ogikubo, Shinya ,  Ohta, Nobuhiro ,  Nakabayashi, Takakazu

IPC分类号： G01N33/84

CPC分类号： G01N33/84 ,  A61B1/043 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/6852 ,  G01N21/6408

摘要： The pH of a substance to be measured is accurately measured without being influenced by the intensity of excitation light (L1), the concentration of fluorescent material and surrounding environments. The pH is measured by generating pulsed excitation-light (L1) including a wavelength that can excite a plurality of kinds of fluorescent-material in living matter that act as coenzyme in oxidation/reduction reaction in vivo, and the intensity of the light not damaging a tissue nor a cell and substantially not changing pH, and by illuminating a predetermined position in the living matter with the light (L1), and by receiving fluorescence (Lf), and by resolving the intensity of the fluorescence (Lf) into time domains the number of which is greater than that of the fluorescent-material, and by detecting the intensities of the fluorescence (Lf) in the respective time domains, and by obtaining approximate-curves having gradients unique to the fluorescent-materials based on the intensities in the respective time domains, and by calculating fluorescence lifetimes (Tf) of at least two of the plurality of kinds of fluorescent-material based on the approximate-curves, and by measuring the pH of the living matter based on the lifetimes (Tf).

摘要翻译： 的物质的被测量测得的pH值被精确地设定，不受激发光的强度（L1），荧光材料和周围环境的浓度的影响。 pH通过产生脉冲激励光（L1），其包括波长测量确实能够激发种荧光材料的的多个生命物质做充当在体内氧化/还原反应的辅酶，并且光的强度不损坏 的组织也不是细胞且基本上不改变pH，并通过照射与光（L1）的生命物质的预定位置，并且通过接收荧光（LF），以及通过解析荧光（LF）的强度成时间区域 的所有的数量是大于使荧光材料制成，并通过在respectivement时间域检测荧光（LF）的强度，并且通过获得具有独特的基于强度的荧光材料梯度近似曲线 所述respectivement时间域，并且通过计算荧光寿命（TF）至少两种基于近似曲线荧光材料的多个的，并且通过测量的pH值 在生命的物质基础上的寿命（TF）。