公开(公告)号：EP3149033A1

公开(公告)日：2017-04-05

申请号：EP15728997.6

申请日：2015-05-28

申请人： Genzyme Corporation

发明人： KODURI, Rao ,  BROWER, Kevin, P. ,  WARIKOO, Veena ,  YU, Marcella ,  HWANG, Christopher ,  KONSTANTINOV, Konstantin ,  YIN, Jin

IPC分类号： C07K16/00 ,  C07K1/113 ,  C08F8/00

CPC分类号： C07K16/2896 ,  A61K39/39591 ,  C07K1/1133 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2839 ,  C07K2317/30 ,  C07K2317/52

摘要： Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

摘要翻译： 本文公开了已经开发用于控制由生物过程产生的多聚体蛋白质的多肽之间形成二硫键的方法。 还公开了允许控制二硫键形成的蛋白质溶液参数。 在一个实例中，本文公开的方法可用于控制抗体溶液中半抗体分子的比例。

公开(公告)号：EP3778884A1

公开(公告)日：2021-02-17

申请号：EP20183936.2

申请日：2014-10-22

申请人： Genzyme Corporation

发明人： LEE, Karen ,  HWANG, Christopher ,  DEMARIA, Christine

IPC分类号： C12N9/40 ,  A61K38/00 ,  A61K38/47 ,  A61P3/00 ,  A61P7/02 ,  A61P9/10 ,  A61P13/12 ,  A61P29/00 ,  A61P43/00 ,  C07K14/705 ,  C12P21/00 ,  A61P9/00

摘要： Provided herein are recombinant glycoproteins (e.g., recombinant human α-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human α-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human α-galactosidase-A protein).

公开(公告)号：EP3237604A2

公开(公告)日：2017-11-01

申请号：EP15843061.1

申请日：2015-12-21

申请人： Genzyme Corporation

发明人： HWANG, Christopher ,  JOHNSON, Timothy ,  WALTHER, Jason ,  CHENG, Cheng ,  WANG, Jonathan ,  SHAH, Neha ,  BAE, Seul-A

IPC分类号： C12N5/00

CPC分类号： C12P21/00 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2500/50 ,  C12N2510/02

摘要： Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

摘要翻译： 本文提供了在液体培养基中培养哺乳动物细胞的方法，所述液体培养基包括浓度为1.8g / L或更高浓度的泊洛沙姆-188或更多的液体培养基或包含选定的泊洛沙姆-188浓度的液体培养基 基于选自以下的一种或多种因素：孔径，孔隙类型，气体流速，培养基中的活细胞密度，以及与细胞应激有关的标记。

公开(公告)号：EP3060660A2

公开(公告)日：2016-08-31

申请号：EP14796346.6

申请日：2014-10-22

申请人： Genzyme Corporation

发明人： LEE, Karen ,  HWANG, Christopher ,  DEMARIA, Christine

IPC分类号： C12N9/40 ,  A61K35/18

CPC分类号： C12N9/2465 ,  A61K9/0019 ,  A61K9/19 ,  A61K38/00 ,  A61K38/47 ,  A61K45/06 ,  C07K14/70592 ,  C12N5/00 ,  C12N2510/00 ,  C12P21/005 ,  C12Y302/01022

摘要： Provided herein are recombinant glycoproteins (e.g., recombinant human α-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human α-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human α-galactosidase-A protein).

摘要翻译： 本文提供具有改变（例如，改进的）糖基化谱的重组糖蛋白（例如，重组人α-半乳糖苷酶-A蛋白）和包括这些蛋白质中的一种或多种的药物组合物和试剂盒。 还提供了产生用于糖蛋白重组表达的哺乳动物细胞的方法（例如，重组人α-半乳糖苷酶-A），产生重组糖蛋白的方法和治疗方法，包括向受试者施用至少一种重组体 糖蛋白（例如，重组人α-半乳糖苷酶-A蛋白）。