公开(公告)号：EP3411476A1

公开(公告)日：2018-12-12

申请号：EP17748280.9

申请日：2017-02-03

Applicant: Trianni, Inc.

Inventor： KILLEEN, Nigel ,  HASENHINDL, Christoph ,  DUONG, Bao

IPC: C12N5/10 ,  A61K35/17 ,  A61K47/62 ,  A61K47/65 ,  A61K39/395 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/08 ,  C40B40/10

CPC classification number: C07K14/315 ,  A01K67/0278 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  C07K14/70503 ,  C07K14/70514 ,  C07K14/70539 ,  C07K14/70596 ,  C07K16/00 ,  C07K16/42 ,  C07K2317/14 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C12N5/0635 ,  C12N5/12 ,  C12N2510/02

Abstract: The present invention provides methods and compositions for generating transgenic animals, including transgenic mammals, as well as plasma cells that allow for cell surface capture of secreted immunoglobulin molecules produced endogenously in the plasma cells.

公开(公告)号：EP3407907A1

公开(公告)日：2018-12-05

申请号：EP17744960.0

申请日：2017-01-27

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： HELMS, Jill ,  DHAMDHERE, Girija ,  LIU, Bo ,  SMITH, Andrew A. ,  GOMEZ, Alan W.

IPC: A61K38/17

CPC classification number: A61K38/18 ,  A61K9/107 ,  C07K14/475 ,  C12N5/0031 ,  C12N2501/415 ,  C12N2510/02

Abstract: Provided herein are methods and culture systems for production of a biologically active Wnt polypeptide under a minimal serum condition. Also described herein include methods and culture systems for production of a biologically active Wnt polypeptide in a serum-free condition.

公开(公告)号：EP3404098A1

公开(公告)日：2018-11-21

申请号：EP17738392.4

申请日：2017-01-10

Applicant: AGC INC.

Inventor： IDIRIS, Alimjan ,  KUMAGAI, Hiromichi ,  KOJIMA, Chiaki

IPC: C12N15/09 ,  C12N1/19 ,  C12P21/00

CPC classification number: C12N15/815 ,  C07K14/79 ,  C12N15/09 ,  C12N15/64 ,  C12N2510/02 ,  C12P21/00 ,  C12P21/02

Abstract: To provide a method for producing transferrin by secretion with high productivity using a transformant of Schizosaccharomyces pombe, and a transformant suitable for the method.
A transformant using Schizosaccharomyces pombe as a host, comprising a transferrin gene and a secretion signal peptide gene expressing transferrin to which a secretion signal peptide which functions in the host binds, located in an upstream region of the transferrin gene, and having Gas2 gene endogenous to the host deleted or inactivated; and a method for producing transferrin, which comprises culturing the transformant in a liquid medium and obtaining transferrin from the liquid medium.

公开(公告)号：EP3384021A1

公开(公告)日：2018-10-10

申请号：EP16805063.1

申请日：2016-11-29

Applicant: European Molecular Biology Laboratory

Inventor： BERGER, Imre ,  LEMKE, Edward ,  KOEHLER, Christine ,  ESTRADA GIRONA, Gemma

IPC: C12N15/09 ,  C12N9/00 ,  C12N15/52 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12P13/00

CPC classification number: C12N9/93 ,  C07K14/47 ,  C07K16/18 ,  C07K16/248 ,  C07K16/32 ,  C07K2317/55 ,  C12N5/0601 ,  C12N9/00 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/67 ,  C12N15/86 ,  C12N2510/02 ,  C12N2710/14143 ,  C12P13/005 ,  C12P21/02 ,  C12Y601/01026

Abstract: The invention relates to a method of preparing engineered target polypeptides (TP) comprising in its amino acid sequence one or more, identical or different, non-canonical amino acid (ncAA) residues, by expressing said TP in an insect cell line (ICL) and by expressing novel orthogonal bacterial aminoacyl tRNA synthetase/tRNA pairs in said ICL; a baculoviral shuttle vector (bacmid) suitable or introducing the genetic information of said orthogonal tRNA synthetase/tRNA into said ILC; particular expression cassettes for expressing said particular tRNAs in said ILC; TPs obtained by said method; as we as a kit for preparing said TPs.

公开(公告)号：EP3353313A2

公开(公告)日：2018-08-01

申请号：EP16778886.8

申请日：2016-09-22

Applicant: Pfizer Inc.

Inventor： HILLER, Gregory Walter ,  MITCHELL, Jeffrey Joseph ,  MULUKUTLA, Bhanu Chandra ,  PEGMAN, Pamela Mary

IPC: C12P21/00 ,  C07K16/00 ,  C07K14/435

CPC classification number: C12P7/62 ,  C07K16/00 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2511/00 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P7/56 ,  C12P17/182

Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

公开(公告)号：EP2351833B1

公开(公告)日：2018-07-18

申请号：EP09823558.3

申请日：2009-10-27

Applicant: Chugai Seiyaku Kabushiki Kaisha

Inventor： KATAYAMA, Satoshi ,  KISHISHITA, Shouhei ,  KODAIRA, Kunihiko ,  SADAMITSU, Makoto ,  TAKAGI, Yoshinori ,  MATSUDA, Hiroki

IPC: C12N5/07 ,  C07K5/062 ,  C07K5/083 ,  C12N5/10 ,  C12P21/02 ,  C07K16/00 ,  C07K5/08

CPC classification number: C12P21/02 ,  C07K5/081 ,  C07K5/0812 ,  C07K16/00 ,  C07K16/303 ,  C07K2317/24 ,  C12N5/0037 ,  C12N2501/998 ,  C12N2510/02

Abstract: Disclosed is a culture medium for culturing an animal cell, which is characterized by containing a peptide comprising, as a constituent unit, an amino acid residue selected from the amino acid group consisting of serine, tyrosine and cysteine. The culture medium is suitable for the high level production of a protein by an animal cell.

公开(公告)号：EP3250583A4

公开(公告)日：2018-07-11

申请号：EP15879438

申请日：2015-01-30

Applicant: CATHAY R&D CENTER CO LTD ,  CATHAY IND BIOTECH LTD

Inventor： CHOU HOWARD ,  LI NAIQIANG ,  LIU XIUCAI

IPC: C07K14/195 ,  C12N5/10 ,  C12N9/88 ,  C12N15/60 ,  C12N15/63 ,  C12N15/70 ,  C12P13/00

CPC classification number: C12N9/88 ,  C12N2510/02 ,  C12P13/001 ,  C12P21/02 ,  C12Y401/01018

Abstract: Provided are lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 and/or fragments thereof. The mutants or fragments have at least 95% sequence identity with SEQ ID NO: 2. Also provided are DNA polynucleotides encoding said lysine decarboxylases, expression vector comprising the DNA polynucleotides, transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.

公开(公告)号：EP3313420A1

公开(公告)日：2018-05-02

申请号：EP16815398.9

申请日：2016-06-24

Applicant: Children's Medical Center Corporation ,  President and Fellows of Harvard College

Inventor： RUBIN, Lee ,  DAVIDOW, Lance ,  ROSSI, Derrick J. ,  EBINA, Wataru ,  STEWART, Morag ,  GUTIERREZ-MARTINEZ, Paula

IPC: A61K35/28 ,  C07K14/495 ,  C12N5/0789

CPC classification number: C12N5/0647 ,  A61K35/28 ,  A61K38/193 ,  C07K14/495 ,  C12N15/86 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2500/62 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2303 ,  C12N2501/2312 ,  C12N2501/26 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2510/02 ,  A61K2300/00

Abstract: The methods and compositions described herein relate to producing, expanding, enriching, and/or maintaining hematopoietic stem cells ex vivo by treating the cells with an agent(s) that exhibits two or more activities selected from modulation of histone methylation; inhibition of TGFβ signaling; inhibition of p38 signaling; activation of canonical Wnt signaling; and modulation of histone acetylation. In some embodiments, the technology described herein relates to transplantation of hematopoietic stem cells.

公开(公告)号：EP2618839B1

公开(公告)日：2018-05-02

申请号：EP11760878.6

申请日：2011-09-20

Applicant: Stichting Wageningen Research

Inventor： KORTEKAAS, Jeroen, Alexander ,  MOORMANN, Robertus, Jacobus, Maria

IPC: A61K39/12 ,  C12N7/00 ,  C07K14/175

CPC classification number: C12N7/00 ,  A61K39/12 ,  C07K14/005 ,  C12N2510/02 ,  C12N2760/12022 ,  C12N2760/12034 ,  C12N2760/12043 ,  C12N2760/12051 ,  C12N2760/12221 ,  C12N2760/12233 ,  C12N2760/12234 ,  Y02A50/397

Abstract: The invention relates to methods of producing infectious bunyavirus replicon particles. These bunyavirus replicon particles are safe and can be used outside a biosafety containment. The invention further relates to recombinant bunyavirus replicon particles and uses of these recombinant bunyavirus replicon particles.

公开(公告)号：EP3207126A4

公开(公告)日：2018-04-04

申请号：EP15850537

申请日：2015-10-09

Applicant: PIONEER HI BRED INT

Inventor： ABAD ANDRE R ,  CROW ANDREW C ,  POLAND BRAD ,  SHI XIAOMEI ,  WOLFE THOMAS C

IPC: C12N15/82 ,  A01H5/10 ,  A01N63/02 ,  C07K14/32 ,  C12N5/04 ,  C12N15/29

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/32 ,  C12N5/04 ,  C12N15/8279 ,  C12N2510/02 ,  C12P21/02

Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.