公开(公告)号：EP2607888A1

公开(公告)日：2013-06-26

申请号：EP11818034.8

申请日：2011-07-27

申请人： Konica Minolta Holdings, Inc.

发明人： TSUKAGOSHI, Masanori ,  KAYA, Takatoshi ,  YAMAMOTO, Noriaki

IPC分类号： G01N21/64 ,  G01N21/05 ,  G01N33/543

CPC分类号： G01N21/6402 ,  G01N21/05 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/54393

摘要： The purpose of the present invention is to provide a sensor area which can prevent the decrease in an assay signal and the increase in an assay blank in an surface plasmon-field enhanced fluorescence spectroscopy (SPFS) measurement. [Solution] The present invention provides an SPFS sensor chip characterized in that a purification area Is arranged upstream of a flow path for an SPFS measurement relative to the direction of flow and a sensor area is arranged downstream of the flow path.

摘要翻译： 本发明的目的是提供一种传感器区域，其可以防止测定信号的降低和表面等离激元增强荧光光谱（SPFS）测量中测定空白的增加。 解决方案本发明提供了一种SPFS传感器芯片，其特征在于，净化区域布置在用于SPFS测量的流动路径的相对于流动方向的上游，并且传感器区域布置在流动路径的下游。

公开(公告)号：EP2579025B1

公开(公告)日：2016-09-28

申请号：EP11792392.0

申请日：2011-06-06

申请人： Konica Minolta Holdings, Inc.

发明人： KAYA, Takatoshi ,  HIKAGE, Naoki ,  NINOMIYA, Hidetaka

IPC分类号： G01N21/64 ,  G01N33/543 ,  C03C17/40

CPC分类号： G01N21/64 ,  C03C17/40 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2610/00

公开(公告)号：EP2579025A1

公开(公告)日：2013-04-10

申请号：EP11792392.0

申请日：2011-06-06

申请人： Konica Minolta Holdings, Inc.

发明人： KAYA, Takatoshi ,  HIKAGE, Naoki ,  NINOMIYA, Hidetaka

IPC分类号： G01N21/64 ,  G01N33/543

CPC分类号： G01N21/64 ,  C03C17/40 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2610/00

摘要： Disclosed is an SPFS sensor chip by which an antigen-antibody reaction or fluorescence can be detected at the maximum efficiency. The SPFS sensor chip, which comprises a transparent substrate, a metal film, an SAM, a solid phase layer having a three-dimensional structure and a ligand, is characterized in that, in said sensor chip, the fluctuation rate represented by the following formula is 0-30% inclusive: [half-width (α)-half width (ss)]/half width (ss)100 [wherein half-width (α) is obtained from a graph that its drawn by plotting the intensity of reflected light of light entering at a definite angle from one surface of sold transparent substrate, said surface not being in contact with the metal film and said reflected light intensity being measured with a light intensity detector placed on the opposite surface side of the transparent substrate, against the angle; and half width (ss) represents the half width of the sensor chip comprising the transparent substrate and the metal film].

摘要翻译： 公开了一种可以以最大效率检测抗原 - 抗体反应或荧光的SPFS传感器芯片。 包括透明基板，金属膜，SAM，具有三维结构的固相和配体的SPFS传感器芯片的特征在于，在所述传感器芯片中，由以下公式表示的波动率 是0-30％的范围：[半宽度（±） - 半宽度（ss）] /半宽度（ss）100 [其中半宽度（±）从图中获得，其通过绘制反射的强度 所述光从所销售的透明基板的一个表面以一定角度进入，所述表面不与金属膜接触，并且所述反射光强度用放置在透明基板的相对表面侧上的光强度检测器测量，抵抗 角度 半宽度（ss）表示包括透明基板和金属膜的传感器芯片的半宽度]。