公开(公告)号：EP0405578A2

公开(公告)日：1991-01-02

申请号：EP90112454.5

申请日：1990-06-29

申请人： NIPPON SHOJI KABUSHIKI KAISHA

发明人： Marui, Yoji ,  Hayashi, Chozo ,  Ito, Shigeki ,  Fujio, Mieko ,  Tanaka, Hiroki ,  Nagasaki, Toshihide ,  Soda, Yasuji ,  Kaneta, Hitoshi

IPC分类号： G01N33/543 ,  G01N33/58

CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/57476 ,  G01N33/581 ,  G01N33/6878

摘要： An enzyme immunoassay for an antigen using a solid phase, which employs (a) a biotinylated antibody in which a biotin derivative is bound to a thiol group of an Fab′ fragment of an antibody, (b) an enzyme-labelled antibody, and (c) a solid phase immobilized with a substance capable of specifically reacting with the above biotin derivative selected from the group consisting of avidin, streptoavidin and a derivative thereof which is bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, or alternatively employs (a′) a solid phase immobilized with a biotinylated antibody via a substance selected from the group consisting of avidin, streptoavidin and a derivative thereof wherein said substance is bound to the biotin moiety of said biotinylated antibody and is also bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, and (b′) an enzyme-­labelled antibody, and a solid phase used therefor.

摘要翻译： 使用固相的抗原的酶免疫测定法，其使用（a）生物素化抗体，其中生物素衍生物与抗体的Fab min片段的硫醇基团结合，（b）酶标记的抗体和（ c）固定有与能够与上述生物素衍生物特异性反应的物质的固相，所述物质选自抗生物素蛋白，链霉亲和素及其衍生物，其直接或通过另一生物素衍生物与高 或者通过选自抗生物素蛋白，链霉亲和素及其衍生物的物质（a min）固定有生物素化抗体的固相，其中所述物质与生物素部分结合， 所述生物素化抗体并且还直接或通过另一种生物素衍生物与结合的高分子量物质之间的连接与固相结合 和（b分钟）酶标记的抗体，以及用于其的固相。

公开(公告)号：EP0405578A3

公开(公告)日：1991-05-29

申请号：EP90112454.5

申请日：1990-06-29

申请人： NIPPON SHOJI KABUSHIKI KAISHA

发明人： Marui, Yoji ,  Hayashi, Chozo ,  Ito, Shigeki ,  Fujio, Mieko ,  Tanaka, Hiroki ,  Nagasaki, Toshihide ,  Soda, Yasuji ,  Kaneta, Hitoshi

IPC分类号： G01N33/543 ,  G01N33/58

CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/57476 ,  G01N33/581 ,  G01N33/6878

摘要： An enzyme immunoassay for an antigen using a solid phase, which employs (a) a biotinylated antibody in which a biotin derivative is bound to a thiol group of an Fab′ fragment of an antibody, (b) an enzyme-labelled antibody, and (c) a solid phase immobilized with a substance capable of specifically reacting with the above biotin derivative selected from the group consisting of avidin, streptoavidin and a derivative thereof which is bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, or alternatively employs (a′) a solid phase immobilized with a biotinylated antibody via a substance selected from the group consisting of avidin, streptoavidin and a derivative thereof wherein said substance is bound to the biotin moiety of said biotinylated antibody and is also bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, and (b′) an enzyme-­labelled antibody, and a solid phase used therefor.

公开(公告)号：EP0405578B1

公开(公告)日：1995-08-30

申请号：EP90112454.5

申请日：1990-06-29

申请人： NIPPON SHOJI KABUSHIKI KAISHA

发明人： Marui, Yoji ,  Hayashi, Chozo ,  Ito, Shigeki ,  Fujio, Mieko ,  Tanaka, Hiroki ,  Nagasaki, Toshihide ,  Soda, Yasuji ,  Kaneta, Hitoshi

IPC分类号： G01N33/543 ,  G01N33/58

CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/57476 ,  G01N33/581 ,  G01N33/6878