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公开(公告)号：EP0243967A3
公开(公告)日：1988-06-15
申请号：EP87106286
申请日：1987-04-30
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
摘要： Nitrile hydration activity of the nitrilase or the immobilized form thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
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公开(公告)号：EP0187680A2
公开(公告)日：1986-07-16
申请号：EP86100305.1
申请日：1986-01-11
摘要： In a process for hydrating and hydrolyzing a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding organic acid, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive Gram-staining microorganism, (b) the microbial cells are allowed to accept light energy of at least about 2 x 10 3 µE/g microbial cells.second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
摘要翻译： 在用于水合和水解由具有微生物腈水解酶活性的腈化合物转变成相应的有机酸的作用的腈化合物的方法，该性能：如产率和反应速度通过用光微生物的照射被显着增强。 本发明的方法的特征在于DASS（a）具有腈水解酶活性的微生物是革兰氏阳性微生物染色，（b）该微生物细胞被允许接受至少约2×10 <3>亩Ë的光能 /水合反应的终止，和（c）前的水合反应是在至少部分地由非透光性的材料构成的容器中进行克微生物cells.second。
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公开(公告)号：EP0187681B1
公开(公告)日：1992-05-20
申请号：EP86100306.9
申请日：1986-01-11
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4.发明公开Process for producing amides by use of microorganisms 失效Verfahren zur Herstellung von Amiden unter Verwendung von Mikroorganismen。
公开(公告)号：EP0187681A2
公开(公告)日：1986-07-16
申请号：EP86100306.9
申请日：1986-01-11
摘要： In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive Gram-straining microorganism, (b) the microbial cells are allowed to accept light energy of about 2×10 -3 to about 1×10 -2 µE/g microbial cells-second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
摘要翻译： 在通过具有腈水解酶活性的微生物的作用将腈化合物水合化以将腈化合物转化成相应的酰胺化合物的方法中，通过用光照射微生物，显着提高了产率和反应速度等性能。 本发明的方法的特征在于（a）具有腈水解酶活性的微生物是正革兰氏菌微生物，（b）允许微生物细胞接受约2×10 -3〜约1×10 3的光能 在水合反应终止之前第二次，和（c）水合反应在至少部分由非透光材料构成的容器中进行。
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公开(公告)号：EP0243967B1
公开(公告)日：1993-12-01
申请号：EP87106286.5
申请日：1987-04-30
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
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公开(公告)号：EP0187680B1
公开(公告)日：1993-04-28
申请号：EP86100305.1
申请日：1986-01-11
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公开(公告)号：EP0187681A3
公开(公告)日：1988-04-13
申请号：EP86100306
申请日：1986-01-11
摘要： In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive Gram-straining microorganism, (b) the microbial cells are allowed to accept light energy of about 2×10 -3 to about 1×10 -2 µE/g microbial cells-second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
摘要翻译： 在通过具有腈水解酶活性的微生物的作用使腈化合物水合以将腈化合物转化成相应的酰胺化合物的过程中，通过用光照射微生物可显着提高产率和反应速度等性能。 本发明的方法的特征在于（a）具有腈水解酶活性的微生物是革兰氏阳性微生物，（b）使微生物细胞接受约2×10-3至约1×10 -2μE/ g微生物细胞 - 在水合反应终止之前 - 和（c）水合反应在至少部分由不透光材料构成的容器中进行。
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公开(公告)号：EP0187680A3
公开(公告)日：1988-03-23
申请号：EP86100305
申请日：1986-01-11
摘要： In a process for hydrating and hydrolyzing a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding organic acid, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive Gram-staining microorganism, (b) the microbial cells are allowed to accept light energy of at least about 2 x 10 3 µE/g microbial cells.second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
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公开(公告)号：EP0243967A2
公开(公告)日：1987-11-04
申请号：EP87106286.5
申请日：1987-04-30
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
摘要： Nitrile hydration activity of the nitrilase or the immobilized form thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
摘要翻译： 通过向腈水解酶或其固定化形式的溶液或悬浮液中添加至少一种选自腈，酰胺和有机酸及其盐的化合物作为稳定剂，腈水解酶或其固定化形式的腈水合活性可以稳定地保存 。
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