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公开(公告)号：EP0243967A3
公开(公告)日：1988-06-15
申请号：EP87106286
申请日：1987-04-30
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
摘要： Nitrile hydration activity of the nitrilase or the immobilized form thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
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公开(公告)号：EP0201039A3
公开(公告)日：1988-12-07
申请号：EP86105958
申请日：1986-04-30
发明人： Umezawa, Hamao , Takeuchi, Tomio , Nagatsu, Toshiharu , Hamada, Masa , Iwadare, Shuichi Banyu Pharm. Co. Ltd. , Matsumoto, Ikuo , Morishima, Hajime
IPC分类号： C12N09/78
CPC分类号： C12N9/80 , C12P13/04 , C12P41/007 , Y10S435/826 , Y10S435/886 , Y10S435/893 , Y10S435/908
摘要： L-aminoacylases S 1 and S 2 derived from actinomycetes and having physicochemical characteristics such that it is a L-aminoacylase which acts on a N-acyl-L-amino acid to give a L-amino acid, its substrate profile is wide, and it acts not only on a N-acyl derivative of a natural L-amino acid, but also on a N-acyl derivative of a synthetic L-amino acid, while it does not act on a N-acyl-D-amino acid, a DL-N-acetyl-a-methylbenzylamine and a N-acetyl-D-glucosamine, etc.
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公开(公告)号：EP0188316A3
公开(公告)日：1988-03-02
申请号：EP86300054
申请日：1986-01-07
发明人： Watanabe, Ichiro , Okumura, Masami
CPC分类号： C12P13/02
摘要： A process for the production of amides utilizing microorganisms is described, which comprises subjecting nitriles having from 2 to 6 carbon atoms to the action of a microorganism belonging to the genus Rhodococcus, the genus Arthrobacter or the genus Microbacterium having an ability to hydrate the nitriles to form the corresponding amides in an aqueous medium.
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公开(公告)号：EP0204555A3
公开(公告)日：1988-03-02
申请号：EP86304254
申请日：1986-06-04
发明人： Kawakami, Kiyoshi , Tanabe, Toyozi , Nagano, Osamu
CPC分类号： C12P13/02
摘要： @ A method of producing an amide comprises subjecting a nitrile to the action of a microorganism belonging to the genus Rhodococcus and having the ability to hydrate the nitrile, and isolating the resulting amide. The micro-organism is highly active to a wide variety of nitrile even at lowtempera- tures and, in addition, the hydration reaction of nitriles has a high selectivity for the corresponding amides. Accordingly, this method ensures the production of amides in high yield without formation of by-products such as corresponding carboxylic acids.
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公开(公告)号：EP0137076A3
公开(公告)日：1986-05-21
申请号：EP83111379
申请日：1983-11-14
发明人： Hideaki, Yamada , Koitchiro, Ryuno
CPC分类号： C12N9/78 , C12N1/20 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase.
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公开(公告)号：EP0115781A3
公开(公告)日：1985-10-23
申请号：EP84100191
申请日：1984-01-10
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one a-amino acid in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
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公开(公告)号：EP0243966A3
公开(公告)日：1988-06-15
申请号：EP87106285
申请日：1987-04-30
摘要： Nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas or Pseudomonas microorganism cells per se or in the immobilized state, all having nitrile hydration activity can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a suspension or solution of the nitrile hydratase, or the microorganism cells per se or in the immobilized state.
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公开(公告)号：EP0188252A3
公开(公告)日：1988-04-13
申请号：EP86100307
申请日：1986-01-11
摘要： In a process for hydrating a nitrile compound by the action of nittrilase produced by a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the nitrilase produced by the microorganism with light. The process of the present invention is characterized in that (a) the nitrilase produced by the microorganism is in the form of crushed cells of the microorganism or intra-cellular substance obtained from the microorganism, (b) the nitrilase produced by the microorganism is allowed to accept light energy of at least about 10 µE/g microbial cells before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
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公开(公告)号：EP0187681A3
公开(公告)日：1988-04-13
申请号：EP86100306
申请日：1986-01-11
摘要： In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive Gram-straining microorganism, (b) the microbial cells are allowed to accept light energy of about 2×10 -3 to about 1×10 -2 µE/g microbial cells-second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light-transmitting material.
摘要翻译： 在通过具有腈水解酶活性的微生物的作用使腈化合物水合以将腈化合物转化成相应的酰胺化合物的过程中，通过用光照射微生物可显着提高产率和反应速度等性能。 本发明的方法的特征在于（a）具有腈水解酶活性的微生物是革兰氏阳性微生物，（b）使微生物细胞接受约2×10-3至约1×10 -2μE/ g微生物细胞 - 在水合反应终止之前 - 和（c）水合反应在至少部分由不透光材料构成的容器中进行。
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10.发明公开Microorganism and plasmid for the constitutive synthesis of creatine amidino hydrolase and method for their production 失效青蒿琥酯水解酶的组成合成的微生物和等离子体及其生产方法
公开(公告)号：EP0187138A3
公开(公告)日：1988-03-23
申请号：EP86100066
申请日：1986-01-03
CPC分类号： C12N9/78 , Y10S435/849 , Y10S435/877
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