公开(公告)号：EP3021867A1

公开(公告)日：2016-05-25

申请号：EP14748051.1

申请日：2014-07-18

申请人： Novartis Tiergesundheit AG

发明人： DEKKER, Brent, E. ,  HERBERT, John, M. ,  REIMNITZ, Michael, J.

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/15 ,  A61K2039/5252 ,  A61K2039/55566 ,  C12N2720/12321 ,  C12N2720/12332 ,  C12N2720/12334 ,  C12N2720/12361

摘要： Disclosed are methods for production of inactivated rotavirus directly from cell culture supernatants containing active or infectious rotavirus (e.g., bovine rotavirus), without prior isolation or purification of the rotavirus from the cell culture supernatants. In some embodiments, inactivated rotavirus (e.g., bovine rotavirus) is produced by heating a volume of culture supernatant to at least a temperature at which the rotavirus is inactivated. In some embodiments, the rotavirus (e.g., bovine rotavirus) is inactivated once the entire volume is heated to at least a temperature at which the rotavirus is inactivated. In some embodiments, the temperature of the cell culture supernatant is maintained for a suitable amount of time, which may be determined using any of the tests described herein. Inactivated rotavirus (e.g., bovine rotavirus) may then be isolated from the culture supernatant.

摘要翻译： 公开了直接从含有活性或感染性轮状病毒（例如牛轮状病毒）的细胞培养上清液中生产灭活轮状病毒的方法，没有事先从细胞培养物上清液中分离或纯化轮状病毒。在一些实施方案中，灭活轮状病毒（例如牛轮状病毒） 通过将体积的培养上清液加热至至少轮状病毒失活的温度而产生。 在一些实施方案中，一旦将整个体积加热至至少轮状病毒灭活的温度，轮状病毒（例如牛轮状病毒）就被灭活。 在一些实施方案中，将细胞培养上清液的温度保持适当的时间，其可以使用本文所述的任何测试来确定。 然后可以从培养物上清液中分离灭活的轮状病毒（例如牛轮状病毒）。