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公开(公告)号：EP1058830A1
公开(公告)日：2000-12-13
申请号：EP99962461.2
申请日：1999-12-24
IPC分类号： G01N15/02
CPC分类号： G01N15/10 , G01N13/04 , G01N33/4905 , G01N2015/1006 , G01N2015/1062 , G01N2015/1087
摘要： The invention relates to a method of analysing a sample of free cells, in particular blood cells. Blood platelet counts are obtained by agitating a blood cell suspension in the presence of air and counting the number of small particles in the suspension both before and after agitation. Cell friability is determined by subjecting a sample of cells to stress in vitro and detecting the number of cell fragments caused by the applied stress. Since applied stress affects red blood cell fragments and platelets differently, it is possible to distinguish between the two types of cells.
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公开(公告)号：EP0870187B1
公开(公告)日：2002-09-11
申请号：EP96944117.9
申请日：1996-12-27
IPC分类号： G01N15/12
CPC分类号： G01N15/12 , G01N2015/0073 , G01N2015/105 , G01N2015/1062 , G01N2015/1087 , G01N2015/1093
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公开(公告)号：EP1144980A2
公开(公告)日：2001-10-17
申请号：EP99962470.3
申请日：1999-12-24
CPC分类号： G01N33/4905
摘要： The present invention measures agglutination using a process which is capable of testing how tightly agglutinated cells are bonded by measuring how much force is required to separate them. By causing red cells which are approximately biconcave discs to sphere, the effective surface area available for bonding diminishes. Sphering a cell increases the space between antigen binding sites and increases the mean distance across which bonding occurs. As the surface area available for bonding between cells decreases as cells sphere they lose bonding strength, thus allowing clumped cells to separate. By recording the inducing pressure and the number of cells (or quantities related to it) as they change with respect to the inducing pressure, agglutination can be detected, quantified and monitored. This provides a simple but effective test for blood grouping and cross-matching by introducing an appropriate source of antibodies and detecting whether or not agglutination occurs.
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公开(公告)号：EP0870184A1
公开(公告)日：1998-10-14
申请号：EP96944116.0
申请日：1996-12-27
CPC分类号： G01N15/10 , G01N13/04 , G01N15/08 , G01N33/483
摘要： In this invention, a measurement of cell permeability is determined by obtaining a measure of the volume of fluid which crosses a sample cell membrane in response to an altered environment. A lytic agent may be used to drive fluid across the cell membranes and thereby cause a change in cell volume. An alteration in osmolality of a sample suspension is preferred, in which the sample suspension is subjected to a continuous osmotic gradient.
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公开(公告)号：EP0870109A1
公开(公告)日：1998-10-14
申请号：EP96944118.0
申请日：1996-12-27
CPC分类号： G01N1/38 , F04B9/047 , G01N35/1095
摘要： A fluid delivery apparatus comprising two or more delivery syringes (17), each delivery syringe comprising a syringe housing (18) defining a tube with a passage having a fluid outlet, and a syringe plunger arranged to slide axially within the tubular passage and seal one end of the tubular passage. The apparatus also includes a syringe plunger drive arranged to drive the syringe plunger along at least part of the length of the tubular passage according to a predetermined velocity profile or the delivery syringe and discharge fluid through the fluid outlet. The predetermined velocity profile of each delivery syringe is such that, in use, a combined flow rate fluid discharge simultaneously from the outlets from the delivery syringes is maintained substantially constant over at least a quarter of the range of movement of each of the plungers. In a preferred example, the syringe plunger drive comprises a rotatable cam (2) and a cam follower (14), being coupled to the syringe plunger to drive the syringe plunger as the cam is rotated.
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公开(公告)号：EP2322910A3
公开(公告)日：2012-02-01
申请号：EP10183397.8
申请日：1999-12-24
CPC分类号： G01N15/10 , G01N13/04 , G01N33/4905 , G01N2015/1006 , G01N2015/1062 , G01N2015/1087
摘要： According to the present invention, there is provided a method of analysing a sample of free cells in vitro comprising the steps of: applying a known or identifiable quantity of stress to the sample; measuring the sample before and after the application of stress to provide at least one reading from which quantitative information relating to the number of cell fragments in the sample caused by the applied stress can be determined; and, relating this reading to the quantity of stress to provide an indication of cell friability.
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公开(公告)号：EP2322910A2
公开(公告)日：2011-05-18
申请号：EP10183397.8
申请日：1999-12-24
CPC分类号： G01N15/10 , G01N13/04 , G01N33/4905 , G01N2015/1006 , G01N2015/1062 , G01N2015/1087
摘要： According to the present invention, there is provided a method of analysing a sample of free cells in vitro comprising the steps of: applying a known or identifiable quantity of stress to the sample; measuring the sample before and after the application of stress to provide at least one reading from which quantitative information relating to the number of cell fragments in the sample caused by the applied stress can be determined; and, relating this reading to the quantity of stress to provide an indication of cell friability.
摘要翻译： 根据本发明，提供了一种在体外分析游离细胞样品的方法，包括以下步骤：向样品施加已知或可识别的应力; 在施加应力之前和之后测量样品以提供至少一个读数，从中可以确定与施加的应力引起的样品中细胞碎片数量有关的定量信息; 并且将该读数与压力的数量相关联以提供细胞脆性的指示。
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公开(公告)号：EP0870184B1
公开(公告)日：2002-07-10
申请号：EP96944116.1
申请日：1996-12-27
IPC分类号： G01N15/10
CPC分类号： G01N15/10 , G01N13/04 , G01N15/08 , G01N33/483
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公开(公告)号：EP0870359B1
公开(公告)日：2000-03-29
申请号：EP96943255.8
申请日：1996-12-27
IPC分类号： H03B28/00
摘要： In a method of generating a clock signal having a desired frequency, a pulse is generated each time a stored accumulator value ( tank ) is found to be greater than or equal to a stored ( trigger ) value. In a first loop (10), the stored accumulator value ( tank ) is iteratively incremented by a first iterative value ( r ) until the stored accumulator value is greater than or equal to the stored trigger value and subsequently in a second loop (11) the stored accumulator value ( tank ) is decremented by a second iterative value until the stored accumulator value is less than the stored trigger value. During each iteration of the first loop (10), a current frequency of the clock signal is compared to a desired frequency value and if the two values are different, the first iterative value ( r ) is corrected at a predetermined rate ( accRate ) over one of more subsequent iterations until the frequency of the generator clock signal corresponds to the detected value of the desired frequency. In a preferred example, the number of iterations needed to change the first iterative value ( r ) is determined by a stored accumulator value which is added to an accelerator-accumulator ( AccTank ) for each iteration that the first iterative value and the desired frequency are not exactly equal.
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公开(公告)号：EP0870186A1
公开(公告)日：1998-10-14
申请号：EP96943256.0
申请日：1996-12-27
IPC分类号： G01N15
CPC分类号： G01N15/1218
摘要： An electrode assembly (1) for resistive pulse spectroscopy comprises two flat electrodes (3) substantially equal in size and shape and supported in face-to-face, parallel relationship ot one another, and a plate-like fluid-tight insulator (2) positioned between the electrodes and extended beyond the periphery of the electrodes with the electrodes (3) being fixed to the insulator (2). Each electrode has a hole (4) extending through the electrode in a central region thereof (4), the holes (4) of the two electrodes (3) being aligned with one another. The insulator (2) has a cavity (5) which receives an initially separate element (40) having a preformed aperture (16) therein which is located substantially centrally with respect to the holes (4) in the electrodes (3) and has a smaller diameter than the said holes, the aperture (16) being located such as to allow a flow of liquid to pass therethrough and through the holes in the electrode.
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