公开(公告)号：EP1966371A1

公开(公告)日：2008-09-10

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP1966371B1

公开(公告)日：2011-08-24

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.