公开(公告)号：EP1966371A1

公开(公告)日：2008-09-10

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP2271675A2

公开(公告)日：2011-01-12

申请号：EP09731098.1

申请日：2009-04-07

申请人： ZymoGenetics, Inc.

发明人： BISHOP, Paul, D. ,  POWNDER, Tracey, A. ,  SHEPPARD, Paul, O. ,  STENLAND, Christopher, J.

IPC分类号： C07K17/00

CPC分类号： C12N9/6418 ,  C07K2319/50

摘要： Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.

公开(公告)号：EP1966371B1

公开(公告)日：2011-08-24

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.