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    PSEUDOTYPED LENTIVIRAL VECTORS
    11.
    发明公开
    PSEUDOTYPED LENTIVIRAL VECTORS 审中-公开

    公开(公告)号：EP3670651A1

    公开(公告)日：2020-06-24

    申请号：EP19202980.9

    申请日：2015-01-09

    申请人： Sirion Biotech GmbH ,  Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH

    发明人： ANASTASOV, Natasa ,  HÖFIG, Ines ,  THIRION, Christian

    IPC分类号： C12N7/00 ,  C12N15/867 ,  C07K14/145 ,  C07K16/28

    摘要： The present invention relates to a nucleic acid molecule comprising or consisting of a nucleic acid sequence encoding the vesicular stomatitis virus envelope glycoprotein (VSV-G) linked to a (poly)peptide comprising or consisting of a cell membrane-binding domain, said nucleic acid sequence comprising in 5' to 3' direction (a) a first sequence segment encoding an endoplasmic reticulum (ER) signal sequence; (b) a second sequence segment encoding said (poly)peptide comprising or consisting of a cell membrane-binding domain; (c) a third sequence segment encoding a linker; and (d) a fourth sequence segment encoding said VSV-G. Further, the invention relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising said vector or nucleic acid molecule, the polypeptide encoded by said nucleic acid molecule and a method of producing the polypeptide encoded by said nucleic acid molecule. In addition, the invention relates to a pseudotyped lentiviral vector particle, a method of transducing a cell as well as a kit comprising various combinations of the nucleic acid molecule, vectors, polypeptides and host cells of the invention.

    DEVICE AND METHOD FOR MASS SPECTROSCOPIC ANALYSIS OF PARTICLES
    14.
    发明公开
    DEVICE AND METHOD FOR MASS SPECTROSCOPIC ANALYSIS OF PARTICLES 审中-公开

    公开(公告)号：EP3567625A1

    公开(公告)日：2019-11-13

    申请号：EP18171596.2

    申请日：2018-05-09

    申请人： Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) ,  Photonion GmbH

    发明人： PASSIG, Dr. Johannes ,  ZIMMERMANN, Dr. Ralf ,  EHLERT, Dr. Sven

    IPC分类号： H01J49/04 ,  H01J49/16

    摘要： The invention relates to a device and a corresponding method for mass spectroscopic analysis of particles, the device comprising: a first irradiation unit (4) configured to irradiate a particle (1) with electromagnetic radiation to cause components of the particle (1) to detach, in particular to desorb, ablate and/or evaporate, from the particle (1), the detached components (2) of the particle (1) being located in proximity of a residual core (3) of the particle (1), a second irradiation unit (14 - 16, 19) configured to irradiate substantially simultaneously i) at least a part of the detached components (2), and optionally the residual core (3) of the particle (1), with a first beam (17) of electromagnetic radiation to cause an ionization of at least a part of the detached components (2), the first beam (17) of electromagnetic radiation exhibiting a first intensity, and ii) at least a part of the residual core (3) of the particle (1) with a second beam (18) of electromagnetic radiation to cause an ionization of at least a part of the components of the residual core (3) of the particle (1), the second beam (18) of electromagnetic radiation exhibiting a second intensity, which is preferably larger than the first intensity, and a mass spectrometer comprising an ion source region (5) configured to accommodate positive ions (+) and/or negative ions (-) of the detached components (2) and/or of the components of the residual core (3), a first detection channel (6) configured to detect the positive ions (+), and optionally a second detection channel (9) configured to detect the negative ions (-).

    PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF
    19.
    发明公开
    PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF 审中-公开

    公开(公告)号：EP3434775A1

    公开(公告)日：2019-01-30

    申请号：EP18181129.0

    申请日：2012-06-06

    申请人： Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

    发明人： KÜHN, Ralf

    IPC分类号： C12N15/62 ,  C12N15/82 ,  C12N15/85 ,  A01K67/027 ,  C07K14/415 ,  C07K14/195 ,  C12N9/16 ,  C12N9/22 ,  C12N15/90

    摘要： The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease. Also, the present invention relates to a vector comprising the nucleic acid molecule and a protein encoded by said nucleic acid molecule. Further, the invention relates to a method of modifying the genome of a eukaryotic cell and a method of producing a non-human vertebrate or mammal.