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14 条记录 (耗时 0.019 秒)

    PSEUDOTYPED LENTIVIRAL VECTORS
    4.
    发明公开
    PSEUDOTYPED LENTIVIRAL VECTORS 审中-公开
    假慢病毒载体

    公开(公告)号：EP3092306A1

    公开(公告)日：2016-11-16

    申请号：EP15700128.0

    申请日：2015-01-09

    申请人： Sirion Biotech GmbH ,  Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

    发明人： ANASTASOV, Natasa ,  HÖFIG, Ines ,  THIRION, Christian

    IPC分类号： C12N7/00 ,  C12N15/867 ,  C07K14/145

    摘要： The present invention relates to a nucleic acid molecule comprising or consisting of a nucleic acid sequence encoding the vesicular stomatitis virus envelope glycoprotein (VSV-G) linked to a (poly)peptide comprising or consisting of a cell membrane-binding domain, said nucleic acid sequence comprising in 5′ to 3′ direction (a) a first sequence segment encoding an endoplasmic reticulum (ER) signal sequence; (b) a second sequence segment encoding said (poly)peptide comprising or consisting of a cell membrane-binding domain; (c) a third sequence segment encoding a linker; and (d) a fourth sequence segment encoding said VSV-G. Further, the invention relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising said vector or nucleic acid molecule, the polypeptide encoded by said nucleic acid molecule and a method of producing the polypeptide encoded by said nucleic acid molecule. In addition, the invention relates to a pseudotyped lentiviral vector particle, a method of transducing a cell as well as a kit comprising various combinations of the nucleic acid molecule, vectors, polypeptides and host cells of the invention.

    RETROVIRAL TRANSDUCTION USING POLOXAMERS
    6.
    发明公开
    RETROVIRAL TRANSDUCTION USING POLOXAMERS 审中-公开
    RETROVIRALE TRANSDUKIT MIT POLOXAMEREN

    公开(公告)号：EP2820138A1

    公开(公告)日：2015-01-07

    申请号：EP13706283.2

    申请日：2013-02-28

    申请人： Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) ,  Sirion Biotech GmbH

    发明人： ANASTASOV, Natasa ,  HÖFIG, Ines ,  THIRION, Christian

    IPC分类号： C12N15/867 ,  A61K48/00

    摘要： The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.

    摘要翻译： 本发明涉及一种转导靶细胞的方法，所述方法包括使靶细胞与逆转录病毒载体和分子量为12.8kDa至约15kDa的泊洛沙姆接触的步骤。 此外，本发明涉及如本文所定义的泊洛沙姆，任选地与本文定义的聚阳离子物质组合用于用逆转录病毒载体转导靶细胞的用途，以及包含逆转录病毒载体，本文定义的泊洛沙姆的试剂盒， 可选择使用说明书。

    PSEUDOTYPED LENTIVIRAL VECTORS
    8.
    发明公开
    PSEUDOTYPED LENTIVIRAL VECTORS 审中-公开

    公开(公告)号：EP3670651A1

    公开(公告)日：2020-06-24

    申请号：EP19202980.9

    申请日：2015-01-09

    申请人： Sirion Biotech GmbH ,  Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH

    发明人： ANASTASOV, Natasa ,  HÖFIG, Ines ,  THIRION, Christian

    IPC分类号： C12N7/00 ,  C12N15/867 ,  C07K14/145 ,  C07K16/28

    摘要： The present invention relates to a nucleic acid molecule comprising or consisting of a nucleic acid sequence encoding the vesicular stomatitis virus envelope glycoprotein (VSV-G) linked to a (poly)peptide comprising or consisting of a cell membrane-binding domain, said nucleic acid sequence comprising in 5' to 3' direction (a) a first sequence segment encoding an endoplasmic reticulum (ER) signal sequence; (b) a second sequence segment encoding said (poly)peptide comprising or consisting of a cell membrane-binding domain; (c) a third sequence segment encoding a linker; and (d) a fourth sequence segment encoding said VSV-G. Further, the invention relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising said vector or nucleic acid molecule, the polypeptide encoded by said nucleic acid molecule and a method of producing the polypeptide encoded by said nucleic acid molecule. In addition, the invention relates to a pseudotyped lentiviral vector particle, a method of transducing a cell as well as a kit comprising various combinations of the nucleic acid molecule, vectors, polypeptides and host cells of the invention.

    AAV-BASED CONDITIONAL EXPRESSION SYSTEM
    9.
    发明公开
    AAV-BASED CONDITIONAL EXPRESSION SYSTEM 审中-公开

    公开(公告)号：EP3408380A1

    公开(公告)日：2018-12-05

    申请号：EP17702844.6

    申请日：2017-01-30

    申请人： Sirion Biotech GmbH

    发明人： KOSZINOWSKI, Ulrich ,  LANGER, Simona ,  RUZSICS, Zsolt ,  THIRION, Christian

    IPC分类号： C12N5/10 ,  C12N15/86 ,  C12Q1/68

    CPC分类号： C12N15/86 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12Q1/6897

    摘要： The present invention relates to a cell comprising (aa) a nucleic acid comprising in 5' to 3' direction (i) at least one adeno-associated virus (AAV) inverted terminal repeat (ITR) sequence; (ii) a promoter which is capable of being activated by (a) helper polypeptide(s) and optionally (a) helper polynucleotide(s); and (iii) a transgenic coding sequence under the control of said promoter of (aa)(ii); and (ab) a nucleic acid comprising in 5' to 3' direction (i) a promoter which is capable of being activated by said helper polypeptide(s) and optionally said helper polynucleotide(s); and (ii) at least one AAV rep gene coding sequence under the control of said promoter of (ab)(i); wherein said cell does not comprise an AAV cap gene and/or is not able to express any AAV cap gene product.