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    Methods for detecting growth factor receptor expression
    14.
    发明公开
    Methods for detecting growth factor receptor expression 失效
    Verfahren zum Nachweis von Wachstumsfaktorrezeptorexpression。

    公开(公告)号：EP0339884A1

    公开(公告)日：1989-11-02

    申请号：EP89303998.2

    申请日：1989-04-21

    申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY

    发明人： Rakowicz-Szulczynska, Ewa

    IPC分类号： G01N33/566 ,  G01N33/567 ,  G01N33/68 ,  G01N33/574

    CPC分类号： G01N33/74 ,  Y10S436/813 ,  Y10S436/824

    摘要： Growth factor receptor expression is detected by formation of a precipitate of polysome-bound receptor and growth factor on room-temperature incubation of mem­brane-free cytoplasm from a tumor cell with excess growth factor if the tumor cell contains actively synthesizing receptor capable of binding to the growth factor. Alter­natively isolated nuclei of a tumor cell are incubated at room temperature with varying concentrations of labelled growth factor in pure membrane-free cytoplasm of the tumor cell, in cytoplasm of the tumor cell con­taining cell membrane, in synthetic medium and in syn­thetic medium containing microsomal fractions from the tumor cell, so as to cause the nuclei to incorporate the labelled growth factor, and the levels of nuclear uptake in each fraction are compared.

    摘要翻译： 如果肿瘤细胞含有主动合成的能够结合到受体的受体的受体，则通过在具有过量生长因子的肿瘤细胞的无膜细胞质的室温培养中形成多聚糖结合受体和生长因子的沉淀来检测生长因子受体表达 生长因子 或者，将分离的肿瘤细胞核在室温下与不同浓度的标记生长因子在肿瘤细胞的纯无膜细胞质，含有细胞膜的肿瘤细胞的细胞质，合成培养基和含有微粒体分数的​​合成培养基中孵育 从肿瘤细胞中引起细胞核结合标记的生长因子，并比较每个部分的核摄取水平。

    Medium for cloning and long-term serial cultivation of human endothelial cells
    16.
    发明公开
    Medium for cloning and long-term serial cultivation of human endothelial cells 失效
    中等Kl ung ung ung。hen hen hen hen。。。。。。。。。。。。。。

    公开(公告)号：EP0142344A2

    公开(公告)日：1985-05-22

    申请号：EP84307747.0

    申请日：1984-11-09

    申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY

    发明人： Levine, Elliot M

    IPC分类号： C12N5/00

    CPC分类号： C12N5/069 ,  C12N2501/90 ,  C12N2501/91

    摘要： Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing a cell culture medium, animal or human serum, endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 population doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

    摘要翻译： 使用含有细胞培养基，动物或人血清，内皮细胞生长因子和肝素和/或硫酸葡聚糖的培养基实现体外人血管内皮细胞的剧烈生长。 获得13〜21个小时的细胞群倍增时间为60〜85次倍增，克隆的人内皮细胞株具有相似的增殖能力。