专利检索 ap:("Centro De Estudios Cientificos De Valdivia" OR "Carnegie Institution Of Washington") AND inv:"FROMMER, Wolf, B." 第 2 页

11.

发明公开
METHODS OF REDUCING REPEAT-INDUCED SILENCING OF TRANSGENE EXPRESSION AND IMPROVED FLUORESCENT BIOSENSORS 审中-公开
标题翻译：异径转基因表达重复诱导的沉默的方法和改进的荧光生物传感器

公开(公告)号：EP1943263A2

公开(公告)日：2008-07-16

申请号：EP05812428.0

申请日：2005-10-14

申请人： CARNEGIE INSTITUTION OF WASHINGTON

发明人： FROMMER, Wolf, B. , DEUSCHLE, Karen

IPC分类号： C07H21/04 , C12N5/00 , C12N15/00 , A01K67/00 , C12Q1/68 , C07K14/00

CPC分类号： C07H21/04 , G01N33/542

摘要： Methods of avoiding repeat- and homology-induced silencing of transgenes are disclosed, in which trangene sequences are genetically altered to reduce the affects of gene silencing. FRET biosensors containing such genetic alterations for improved expression in cell lines and in vivo are disclosed.

12.

发明公开
ENVIRONMENTALLY STABLE SENSORS AND METHODS OF USING THE SAME 审中-公开
标题翻译：环境传感器稳定和申请流程。

公开(公告)号：EP1856138A1

公开(公告)日：2007-11-21

申请号：EP05811873.8

申请日：2005-10-14

申请人： CARNEGIE INSTITUTION OF WASHINGTON

发明人： FROMMER, Wolf, B. , LOGER, Loren

IPC分类号： C07H21/04

CPC分类号： G01N33/54373 , A01K2217/05 , A01K2227/703 , A01K2267/0393 , C07K14/195 , C07K2319/24 , C07K2319/60 , C12N15/8509 , C12Q1/02 , G01N33/5008 , G01N33/502 , G01N33/542

摘要： Environmentally stable biosensors are disclosed, which comprise a ligand binding domain from a thermophilic organism conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon ligand binding. Such biosensors demonstrate enhanced acid-, thermal- and chemical stability as compared to sensors constructed using protein domains from mesophilic organisms.

13.

发明公开
DEVELOPMENT OF SENSITIVE FRET SENSORS AND METHODS OF USING THE SAME 审中-公开
标题翻译：敏感性FRET传感器及其使用方法的发展

公开(公告)号：EP1815246A2

公开(公告)日：2007-08-08

申请号：EP05812048.6

申请日：2005-10-14

申请人： CARNEGIE INSTITUTION OF WASHINGTON

发明人： FROMMER, Wolf, B. , OKUMOTO, Sakiko , LOOGER, Loren , FEHR, Marcus

IPC分类号： G01N33/50

CPC分类号： G01N33/566 , C07K14/245 , C12Q1/6818 , G01N33/542 , G01N2800/2814 , G01N2800/2835 , G01N2800/2857 , G01N2800/2871

摘要： Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.