公开(公告)号：EP0786086A1

公开(公告)日：1997-07-30

申请号：EP95933318.0

申请日：1995-09-30

申请人： FORSCHUNGSZENTRUM JÜLICH GMBH

发明人： THUST, Marion ,  SCHÖNING, Michael, Josef ,  VETTER, Joachim ,  KAUPP, Ulrich, Benjamin ,  KORDOS, Peter ,  LÜTH, Hans

IPC分类号： G01N33 ,  C07K17 ,  C12N9 ,  C12N11

CPC分类号： C12N11/14 ,  C12N9/86 ,  G01N33/54353 ,  G01N33/551 ,  G01N33/552 ,  G01N2415/00

摘要： In order to immobilise bio-material, e.g. enzymes, micro-organisms, cells, organelles, etc., on a substrate with a Si3N4 surface with bonding-active NHx groups, use is made of a hetero-functional cross-linking agent with a bio-material coupling function on the one hand and a NHx-reactive group on the other. The immobilisation substrate preferably takes the form of a 10-1000 mm thick Si3N4 layer which is separated from SiH4/NH3 by CVD and coated with bonding-active NHx groups by hydrolysing surface cleaning, especially with dilute acid. Advantageously, a hetero-bifunctional cross-linking agent is first caused to react with an NH2-reactive aldehyde, ester, halide, epoxide, imine or isocyanate group and, after the removal of unbonded superfluous cross-linking agent, coupled to the biomaterial by means of the bio-reactive group.