MESSVERFAHREN UNTER EINSATZ MINDESTENS EINER BIOLOGISCHEN REZEPTORZELLE SOWIE EINE ZUR DURCHFÜHRUNG DES MESSVERFAHRENS GEEIGNETE VORRICHTUNG
    1.
    发明授权
    MESSVERFAHREN UNTER EINSATZ MINDESTENS EINER BIOLOGISCHEN REZEPTORZELLE SOWIE EINE ZUR DURCHFÜHRUNG DES MESSVERFAHRENS GEEIGNETE VORRICHTUNG 有权
    测量方法使用至少一个生物受体细胞以及实施测量器件非常适合的一种方法

    公开(公告)号:EP1255982B1

    公开(公告)日:2005-11-09

    申请号:EP00989805.7

    申请日:2000-11-28

    IPC分类号: G01N27/414

    CPC分类号: G01N33/5005 G01N33/0031

    摘要: The reactions of single cells to particular substances in their environment can be established by means of signal converters. In a manner known per se, the potential difference between the interior and the exterior of a cell is measured by means of electrodes, whereby the cell is, however, damaged at least in certain places. Conventional methods which permit a recording of a cell reaction without intervening in the cell interior are either complex or limited in response. In a measuring technique, using at least one biological receptor cell, an asymmetric, extra-cellular, electrical potential of the at least one receptor cell is formed, which produces a multi-pole field and said multi-pole field is applied to determining the cell reaction. The asymmetry in the extra-cellular electrical potential of the at least one receptor cell which reacts to environmental changes can be achieved by, for example, embedding the at least one receptor cell in a bedding element which corresponds to the geometrical form thereof.

    BIOSENSOR MIT AUF EINER SIGNALAKTIVEN OBERFLÄCHE KOVALENT FIXIERTEM BIOMATERIAL
    3.
    发明公开
    BIOSENSOR MIT AUF EINER SIGNALAKTIVEN OBERFLÄCHE KOVALENT FIXIERTEM BIOMATERIAL 失效
    随着对SIGNAL活性表面共价固定生物材料生物传感器

    公开(公告)号:EP0784791A1

    公开(公告)日:1997-07-23

    申请号:EP95933316.0

    申请日:1995-09-30

    IPC分类号: C12M1 C12Q1 G01N27 G01N33

    CPC分类号: G01N33/54373 C12Q1/001

    摘要: The successful bonding of bioactive material, especially enzymes, to the signal-active surface of a biosensor can be achieved by means of a heterobifunctional crosslinking agent having a group A reactive for the signal-active surface, e.g. a group reacting with -OH, -SH or -NHx, and a photoactivatable, biomaterial-reactive group B. Known groups B are, in particular, azidophenyl and azidosalicylic groups. Crosslinking agents containing such groups B on the one hand and succinimide ester groups on the other are commercially available and hence particularly attractive. The signal-active surface can be especially a Si3N4 surface with reactive NHx groups, as can be obtained from the gas phase by SiH4/NH3 reaction, especially on a fresh SiO2 surface, and has been freed from oxides especially by treatment with dilute hydrofluoric acid.

    MESSVERFAHREN UNTER EINSATZ MINDESTENS EINER BIOLOGISCHEN REZEPTORZELLE SOWIE EINE ZUR DURCHFÜHRUNG DES MESSVERFAHRENS GEEIGNETE VORRICHTUNG
    8.
    发明公开
    MESSVERFAHREN UNTER EINSATZ MINDESTENS EINER BIOLOGISCHEN REZEPTORZELLE SOWIE EINE ZUR DURCHFÜHRUNG DES MESSVERFAHRENS GEEIGNETE VORRICHTUNG 有权
    测量方法使用至少一个生物受体细胞以及实施测量器件非常适合的一种方法

    公开(公告)号:EP1255982A2

    公开(公告)日:2002-11-13

    申请号:EP00989805.7

    申请日:2000-11-28

    IPC分类号: G01N27/414

    CPC分类号: G01N33/5005 G01N33/0031

    摘要: The reactions of single cells to particular substances in their environment can be established by means of signal converters. In a manner known per se, the potential difference between the interior and the exterior of a cell is measured by means of electrodes, whereby the cell is, however, damaged at least in certain places. Conventional methods which permit a recording of a cell reaction without intervening in the cell interior are either complex or limited in response. In a measuring technique, using at least one biological receptor cell, an asymmetric, extra-cellular, electrical potential of the at least one receptor cell is formed, which produces a multi-pole field and said multi-pole field is applied to determining the cell reaction. The asymmetry in the extra-cellular electrical potential of the at least one receptor cell which reacts to environmental changes can be achieved by, for example, embedding the at least one receptor cell in a bedding element which corresponds to the geometrical form thereof.

    BIOSENSOR MIT AUF EINER SIGNALAKTIVEN OBERFLÄCHE KOVALENT FIXIERTEM BIOMATERIAL
    10.
    发明授权
    BIOSENSOR MIT AUF EINER SIGNALAKTIVEN OBERFLÄCHE KOVALENT FIXIERTEM BIOMATERIAL 失效
    随着对SIGNAL活性表面共价固定生物材料生物传感器

    公开(公告)号:EP0784791B1

    公开(公告)日:2001-11-21

    申请号:EP95933316.2

    申请日:1995-09-30

    CPC分类号: G01N33/54373 C12Q1/001

    摘要: The successful bonding of bioactive material, especially enzymes, to the signal-active surface of a biosensor can be achieved by means of a heterobifunctional crosslinking agent having a group A reactive for the signal-active surface, e.g. a group reacting with -OH, -SH or -NHx, and a photoactivatable, biomaterial-reactive group B. Known groups B are, in particular, azidophenyl and azidosalicylic groups. Crosslinking agents containing such groups B on the one hand and succinimide ester groups on the other are commercially available and hence particularly attractive. The signal-active surface can be especially a Si3N4 surface with reactive NHx groups, as can be obtained from the gas phase by SiH4/NH3 reaction, especially on a fresh SiO2 surface, and has been freed from oxides especially by treatment with dilute hydrofluoric acid.