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18 条记录 (耗时 0.025 秒)

    PLANT EPSP SYNTHASES AND METHODS OF USE
    12.
    发明公开
    PLANT EPSP SYNTHASES AND METHODS OF USE 无效

    公开(公告)号：EP3356535A1

    公开(公告)日：2018-08-08

    申请号：EP16779303.3

    申请日：2016-09-29

    申请人： Pioneer Hi-Bred International, Inc.

    发明人： DONG, Yuxia ,  LASSNER, Michael ,  LU, Jian ,  NG, Emily ,  PATTEN, Phillip A. ,  SIEHL, Daniel

    IPC分类号： C12N15/82 ,  C12N9/10

    CPC分类号： C12N15/8275 ,  C12N9/1092 ,  C12Y205/01019

    摘要： Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

    METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS
    13.
    发明公开
    METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS 审中-公开
    VERFAHREN ZUR HERSTELLUNG EINES KOMPLEXEN TRANSGENEN TRAIT LOCUS

    公开(公告)号：EP2689019A2

    公开(公告)日：2014-01-29

    申请号：EP12712040.0

    申请日：2012-03-22

    申请人： Pioneer Hi-Bred International Inc. ,  E.I. Du Pont De Nemours and Company

    发明人： LASSNER, Michael ,  JANTZ, Derek ,  SMITH, James, Jefferson ,  CIGAN, Mark ,  FALCO, Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-bin ,  SVITASHEV, Sergei

    IPC分类号： C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  C12N15/55

    CPC分类号： C12N15/8202 ,  A01H1/06 ,  C12N9/22 ,  C12N15/8213

    摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

    摘要翻译： 公开了一种在植物中生产复合转基因性状基因座的方法，该基因座包含至少两个在目的基因组区域中改变的靶序列。 所述方法包括使用两种或更多种双链断裂诱导剂，其中每一种可引起目的基因组区域中的靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES
    14.
    发明公开
    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES 审中-公开
    组合物和方法对抑制靶多核苷酸

    公开(公告)号：EP2247732A1

    公开(公告)日：2010-11-10

    申请号：EP09702355.0

    申请日：2009-01-15

    申请人： Pioneer Hi-Bred International Inc.

    发明人： LASSNER, Michael

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8286 ,  C12N15/8218 ,  Y02A40/162

    摘要： Methods and compositions which increase the concentration of an inhibitory RNA specific for a target sequence in a cell are provided. In one embodiment, the methods and compositions employ a first polynucleotide comprising a silencing element for a target pest sequence operably linked to a promoter active in the plant cell; and, a second polynucleotide comprising a suppressor enhancer element comprising the target pest sequence or an active fragment or variant thereof operably linked to a promoter active in the plant cell. The combined expression of the silencing element with the target pest sequence, or an active variant or fragment thereof, leads to the amplification of the inhibitory RNA produced from the silencing element over the achievable with only the expression of the silencing element alone. Thus, the various methods and compositions of the invention provide improved methods for the delivery of inhibitory RNA to a target organism.

    METHODS AND COMPOSITIONS OF IMPROVED PLANT TRANSFORMATION
    16.
    发明公开
    METHODS AND COMPOSITIONS OF IMPROVED PLANT TRANSFORMATION 审中-公开

    公开(公告)号：EP3371313A1

    公开(公告)日：2018-09-12

    申请号：EP16763143.1

    申请日：2016-08-26

    申请人： Pioneer Hi-Bred International, Inc.

    发明人： ANAND, Ajith ,  BASS, Steven Henry ,  CHO, Hyeon-Je ,  KLEIN, Theodore Mitchell ,  LASSNER, Michael ,  MCBRIDE, Kevin E

    IPC分类号： C12N15/82 ,  C07K14/195

    CPC分类号： C12N15/8202 ,  C07K14/195 ,  C12N15/8205

    摘要： The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11, virC1-C2 or r-virC1-C2, virD1-D2 or r-virD1-D2, and virG or r-virG, or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11, r-virC1-C2, r-virD1-D2, and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

    METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI
    18.
    发明公开
    METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI 有权
    程序植物之前病原真菌保护

    公开(公告)号：EP1781795A2

    公开(公告)日：2007-05-09

    申请号：EP05857490.6

    申请日：2005-06-30

    申请人： Pioneer-Hi-Bred International, Inc.

    发明人： LASSNER, Michael ,  WU, Gusui ,  MULLER, Mathias L. ,  KEENAN, Robert J. ,  ALI, Hana

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8282

    摘要： Methods for protecting a plant from a plant pathogenic fungus are provided. A method for enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.