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1026 条记录 (耗时 0.021 秒)

    RNA EXPRESSION REGULATION OR EDITING METHOD THROUGH ACTIVITY CONTROL OF CAS13 PROTEIN
    12.
    发明公开
    RNA EXPRESSION REGULATION OR EDITING METHOD THROUGH ACTIVITY CONTROL OF CAS13 PROTEIN 审中-公开

    公开(公告)号：EP4141112A1

    公开(公告)日：2023-03-01

    申请号：EP22773389.6

    申请日：2022-07-08

    申请人： Korea Advanced Institute of Science and Technology

    发明人： HEO, Won Do ,  YU, Jeonghye

    IPC分类号： C12N9/22 ,  C12N13/00 ,  C12N15/113 ,  C12N15/10 ,  C12N15/63

    摘要： The present invention relates to a RNA expression modulating or editing method through chemical or optogenetic regulation of Cas13 protein activity. Specifically, in order to regulate the activity of Cas13 in the CRISPR-Cas13 system, a fragment of the Cas13 protein was generated to enable recombination, and a chemogenetically or optogenetically bindable protein was linked to each fragment. And, it was confirmed that the Cas13 protein can be activated by treatment of a low molecular weight compound or irradiation of light. Accordingly, the method of the present invention can be effectively used for disease treatment by regulating RNA expression or editing RNA mutations related to disease.

    METHOD AND SYSTEM FOR THE IDENTIFICATION OF OPTIMIZED TREATMENT CONDITIONS
    13.
    发明公开
    METHOD AND SYSTEM FOR THE IDENTIFICATION OF OPTIMIZED TREATMENT CONDITIONS 审中-公开

    公开(公告)号：EP4105312A1

    公开(公告)日：2022-12-21

    申请号：EP21180081.8

    申请日：2021-06-17

    申请人： Bühler AG

    发明人： Buchmann, Leandro

    IPC分类号： C12M1/00 ,  C12M1/42 ,  C12N13/00 ,  G16B40/00

    摘要： The present invention is related to a method for the identification of optimized conditions for treating cells with electric pulses for targeted inactivation, the extraction of bioactive compounds, and the stimulation of cell growth and/or cellular compounds, comprising the steps of treating samples comprising cellular material under at least one condition, preferably two or more different conditions, analysing the results of the treatment in step a) for each of the applied different conditions, and identifying suitable conditions from the analysis of step b). The present invention is furthermore related to a system (1) for performing said method, as well as to a method for treating cells for targeted inactivation, the extraction of bioactive compounds, and the stimulation of cell growth and/or cellular compounds, employing the above identified suitable conditions.