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79 条记录 (耗时 0.028 秒)

    Chimeric plasmid vector
    47.
    发明公开
    Chimeric plasmid vector 失效
    Chimerer Plasmidvektor。

    公开(公告)号：EP0162725A2

    公开(公告)日：1985-11-27

    申请号：EP85303682.0

    申请日：1985-05-24

    申请人： KABUSHIKI KAISHA YAKULT HONSHA

    发明人： Ishiwa, Hiromi ,  Shibahara, Harue ,  Mutai, Masahiko

    IPC分类号： C12N15/74

    CPC分类号： C12N15/74 ,  C12N15/75

    摘要： A chimeric plasmid vector contains (a) a tetracycline resistance gene region derived from the plasmid pAMal of Streptococcus faecalis, (b) an ampicillin resistance gene region derived from the vector pACYC177 of Escherichia coli, (c) a DNA replication origin derived from said plasmid pAMal, (d) a DNA replication origin derived from said vector pACYC177, and (e) a polylinker region having a recognition and cleavage site for the restriction enzyme EcoRI at one terminal and a recognition and cleavage site for the restriction enzyme HindIII at the other terminal of the polylinker region, the plasmid vector having unique recognition and cleavage sites for the restriction enzymes Ba/I, Hpal and EcoRV, which sites are located in said tetracycline resistance gene region, and the plasmid vector having unique recognition and cleavage sites for the restriction enzymes Pvul, Bg/l and BanI, which sites are located in said ampicillin resistance gene region. The vector is useful for conveying a gene into a host cell, usually of a bacterium, and the host can then be employed in biosynthesis of industrial products.

    摘要翻译： 嵌合质粒载体含有（a）源自粪链球菌的质粒pAMα1的四环素抗性基因区，（b）来源于大肠杆菌载体pACYC177的氨苄青霉素抗性基因区，（c）衍生自 所述质粒pAMα1，（d）衍生自所述载体pACYC177的DNA复制起点，和（e）在一个末端具有限制酶EcoRI的识别和切割位点的多接头区，以及限制酶的识别和切割位点 HindIII在多接头区域的另一个末端，该质粒载体具有针对限制酶BalI，HpaI和EcoRV的独特识别和切割位点，该位点位于所述四环素抗性基因区域中，质粒载体具有独特的识别和切割位点 对于限制酶PvuI，BglI和BanI，其位点位于所述氨苄青霉素抗性基因区域中。 该载体可用于将基因输送到通常为细菌的宿主细胞中，然后可将宿主用于工业产品的生物合成。

    A method of introducing a donor DNA into a strain of the genus Lactobacillus
    49.
    发明公开
    A method of introducing a donor DNA into a strain of the genus Lactobacillus 失效
    在einen Stamm der Gattung Lactobacillus的Verfahren zurEinführungvon Spender-DNS。

    公开(公告)号：EP0133046A2

    公开(公告)日：1985-02-13

    申请号：EP84305178.0

    申请日：1984-07-30

    申请人： KABUSHIKI KAISHA YAKULT HONSHA

    发明人： Kadota, Mariko ,  Kudo, Satoshi ,  Mutai, Masahiko

    IPC分类号： C12N15/00 ,  A23C9/123

    CPC分类号： C12N15/746 ,  A23C9/1234 ,  C12N15/88

    摘要： A method of introducing a donor DNA into a strain belonging to the genus Lactobacillus, comprises preparing spheroplasts of the strain, encapsulating donor DNA within liposomes to obtain DNA-encapsulating-liposomes, and allowing the DNA-encapsulating-liposomes to fuse with the spheroplasts in the presence of a fusion inducing agent such as polyethylene glycol. The donor DNA may be provided in the form of recombinant DNA molecules prepared as a vector having inserted therein suitable phage DNA, plasmid DNA or heterogenic DNA retaining useful genetic information. Examples of the phage DNA include the DNA of the virulent phage J1 and the DNA of the temperate phage φFSW selectively retained in the genus L. casei.

    摘要翻译： 将供体DNA引入属于乳杆菌属的菌株的方法包括制备菌株的原生质球，将供体DNA包封在脂质体内以获得DNA封装的脂质体，并使DNA-包封的脂质体与原生质球融合 存在聚乙二醇等融合诱导剂。 供体DNA可以以作为载体插入合适的噬菌体DNA，质粒DNA或保留有用遗传信息的异源DNA的载体制备的重组DNA分子的形式提供。 噬菌体DNA的实例包括有毒噬菌体J1的DNA和选择性保留在干酪乳杆菌属中的温带噬菌体phi FSW的DNA。