公开(公告)号：EP0133046A3

公开(公告)日：1986-07-30

申请号：EP84305178

申请日：1984-07-30

申请人： KABUSHIKI KAISHA YAKULT HONSHA

发明人： Kadota, Mariko ,  Kudo, Satoshi ,  Mutai, Masahiko

IPC分类号： C12N15/00 ,  A23C09/123

摘要： A method of introducing a donor DNA into a strain belonging to the genus Lactobacillus, comprises preparing spheroplasts of the strain, encapsulating donor DNA within liposomes to obtain DNA-encapsulating-liposomes, and allowing the DNA-encapsulating-liposomes to fuse with the spheroplasts in the presence of a fusion inducing agent such as polyethylene glycol. The donor DNA may be provided in the form of recombinant DNA molecules prepared as a vector having inserted therein suitable phage DNA, plasmid DNA or heterogenic DNA retaining useful genetic information. Examples of the phage DNA include the DNA of the virulent phage J1 and the DNA of the temperate phage φFSW selectively retained in the genus L. casei.

摘要翻译： 将供体DNA引入属于乳酸杆菌属的菌株的方法包括制备菌株的原生质球，将供体DNA包封在脂质体内以获得DNA包封脂质体，并使DNA包封脂质体与原生质球中的原生质融合 融合诱导剂如聚乙二醇的存在。 供体DNA可以以制备为载体的重组DNA分子形式提供，所述载体具有插入其中的合适噬菌体DNA，质粒DNA或保留有用遗传信息的异源DNA。 噬菌体DNA的实例包括强毒噬菌体J1的DNA和选择性保留在干酪乳杆菌属的温和噬菌体φFSW的DNA。

公开(公告)号：EP0133046A2

公开(公告)日：1985-02-13

申请号：EP84305178.0

申请日：1984-07-30

申请人： KABUSHIKI KAISHA YAKULT HONSHA

发明人： Kadota, Mariko ,  Kudo, Satoshi ,  Mutai, Masahiko

IPC分类号： C12N15/00 ,  A23C9/123

CPC分类号： C12N15/746 ,  A23C9/1234 ,  C12N15/88

摘要： A method of introducing a donor DNA into a strain belonging to the genus Lactobacillus, comprises preparing spheroplasts of the strain, encapsulating donor DNA within liposomes to obtain DNA-encapsulating-liposomes, and allowing the DNA-encapsulating-liposomes to fuse with the spheroplasts in the presence of a fusion inducing agent such as polyethylene glycol. The donor DNA may be provided in the form of recombinant DNA molecules prepared as a vector having inserted therein suitable phage DNA, plasmid DNA or heterogenic DNA retaining useful genetic information. Examples of the phage DNA include the DNA of the virulent phage J1 and the DNA of the temperate phage φFSW selectively retained in the genus L. casei.

摘要翻译： 将供体DNA引入属于乳杆菌属的菌株的方法包括制备菌株的原生质球，将供体DNA包封在脂质体内以获得DNA封装的脂质体，并使DNA-包封的脂质体与原生质球融合 存在聚乙二醇等融合诱导剂。 供体DNA可以以作为载体插入合适的噬菌体DNA，质粒DNA或保留有用遗传信息的异源DNA的载体制备的重组DNA分子的形式提供。 噬菌体DNA的实例包括有毒噬菌体J1的DNA和选择性保留在干酪乳杆菌属中的温带噬菌体phi FSW的DNA。