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公开(公告)号：EP2654915B1
公开(公告)日：2018-10-24
申请号：EP11850413.3
申请日：2011-12-19
发明人： BJÖRKMAN, Tomas , MONIE, Elin , RODRIGO, Gustav
IPC分类号： B01D15/38 , B01J20/281 , C07K1/22 , C07K14/745 , C12N15/03 , B01J20/32 , B01J20/286 , B01J20/289 , C07K14/31
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/745
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
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公开(公告)号：EP3341119A1
公开(公告)日：2018-07-04
申请号：EP16757842.6
申请日：2016-08-18
CPC分类号： B01D15/3809 , B01J20/24 , B01J20/28004 , B01J20/28011 , B01J20/28019 , B01J20/286 , B01J20/3212 , B01J20/3274 , B01J20/3278 , B01J20/3293 , B01J2220/603 , C07K1/22 , C07K16/065
摘要： The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.
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公开(公告)号：EP2654914B1
公开(公告)日：2018-05-30
申请号：EP11850306.9
申请日：2011-12-19
IPC分类号： B01D15/38 , B01J20/281 , C07K1/22 , C07K14/31 , C12N15/03 , B01J20/289 , B01J20/286 , B01J20/32
CPC分类号： C07K16/1271 , B01D15/168 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine or Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
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公开(公告)号：EP2337632B1
公开(公告)日：2018-04-11
申请号：EP09820836.6
申请日：2009-10-07
发明人： LACKI, Karol
CPC分类号： B01L3/50255 , B01L3/5085 , B01L2300/069 , G01N30/466 , G01N30/6043 , G01N30/8658 , Y10T436/25
摘要： A micro titer plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.
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公开(公告)号：EP3265198A1
公开(公告)日：2018-01-10
申请号：EP16706227.2
申请日：2016-02-25
发明人： MONIE, Elin, Marianne , BJORKMAN, Tomas , GRONBERG, Anna , LJUNGLOF, Anders , RODRIGO, Gustav, Jose , TORSTENSON, Karin , WETTERHALL, Magnus, Carl, Erik
CPC分类号： A61L2/18 , B01D15/203 , B01D15/3804 , G01N30/50
摘要： The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of: a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support, b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I, R—O—O—H (I) wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.
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公开(公告)号：EP2117685B1
公开(公告)日：2017-08-02
申请号：EP08715706.1
申请日：2008-02-07
发明人： RUDSTEDT, Asa , AXEN, Andreas , NORRMAN, Nils , LARSSON, Anders
CPC分类号： B01D71/10 , B01D15/36 , B01D15/3804 , B01D67/0093 , B01D71/16 , B01D2323/30 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28085 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3244
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公开(公告)号：EP2139596B1
公开(公告)日：2017-06-21
申请号：EP08741857.0
申请日：2008-04-21
发明人： MA, Guang-Hui , SU, Zhi-Guo , ZHOU, Qing-Zhu
IPC分类号： B01J20/285 , B01J20/291
CPC分类号： B01J13/0065 , B01D15/34 , B01J20/262 , B01J20/28004 , B01J20/28019 , B01J20/285 , B01J20/291 , Y10T428/2989
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公开(公告)号：EP1972689B1
公开(公告)日：2017-06-07
申请号：EP08004374.8
申请日：2003-03-20
发明人： Hober, Sophia
CPC分类号： B01D15/3809 , B01J20/24 , B01J20/286 , B01J20/289 , B01J20/3244 , B01J2220/44 , B01J2220/52 , C07K1/22 , C07K14/001 , C07K14/195 , C07K14/31 , C07K16/065 , Y10S530/81 , Y10S530/825
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公开(公告)号：EP3074106A1
公开(公告)日：2016-10-05
申请号：EP14866010.3
申请日：2014-11-26
IPC分类号： B01D15/00 , B01J20/26 , B01J20/285 , B01J20/32 , C12H1/04
CPC分类号： C12H1/0424 , B01D15/00 , B01J20/28004 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/327 , B01J20/3278 , B01J20/3293 , B01J2220/58 , C08F251/00
摘要： The invention discloses a separation matrix witha porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which compriseat least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.
摘要翻译： 本发明公开了一种具有多孔固体支持物和共价连接到固体支持物上的多个聚乙烯吡咯烷酮（PVP）聚合物链的分离基质。 聚乙烯吡咯烷酮聚合物链是包含至少70mol％乙烯基吡咯烷酮单体残基和小于2mol％带负电荷的单体残基的乙烯基吡咯烷酮均聚物链或共聚物链。
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公开(公告)号：EP2931400A4
公开(公告)日：2016-08-24
申请号：EP13862375
申请日：2013-12-12
发明人： LACKI KAROL , JOHANSSON HANS J
CPC分类号： B01D15/20 , B01D15/3809 , C07K1/16 , G01N30/50 , G01N2030/8813 , Y02P20/582
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