公开(公告)号：EP1712917A4

公开(公告)日：2007-08-01

申请号：EP03786278

申请日：2003-12-24

申请人： DENKA SEIKEN KK

发明人： SHIMIZU HIDEHARU ,  WATANABE TAKESHI ,  TAKIZAWA KAZUYUKI ,  MATSUDA JUNJI ,  SATO TOSHINORI

IPC分类号： G01N33/569 ,  B01L3/00 ,  G01N1/10 ,  G01N1/28 ,  G01N1/34 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N1/4077 ,  B01L3/5023 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0406 ,  G01N33/569 ,  G01N2001/4016 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： A simple membrane assay method wherein an analyte of test sample is assayed with the use of an assay unit including a membrane having, bonded thereto, a trapping reagent for capturing the analyte, which method is characterized in that a test sample is filtered through a filter and thereafter dropped on the membrane to thereby detect the presence of analyte in the test sample. There is further provided a simple membrane assay kit for checking the presence of analyte in a test sample, which assay kit comprises (1) a filter and (2) an assay unit including a membrane having, bonded thereto, a trapping agent for capturing the analyte. Thus, there can be realized a simple test for rapidly checking on site any analyte, such as a pathogenic organism or an antibody, in a test sample collected from medical spots and individuals, in which the occurrence of false positive is prevented, enabling high-precision detection of the analyte.