公开(公告)号：EP3045913A4

公开(公告)日：2017-05-31

申请号：EP14843481

申请日：2014-09-10

申请人： DENKA SEIKEN KK

发明人： SHINOHARA YUKI ,  MIYAZAWA TAKASHI

IPC分类号： G01N33/543 ,  G01N33/569

CPC分类号： G01N33/56983 ,  G01N2333/11

摘要： Means for enabling an immunoassay with a sufficient sensitivity in an immunoassay for measuring influenza virus in a sample using influenza virus M1 protein as an antigen is provided. A sample processing method in an immunoassay for influenza virus, which method comprises, in an immunoassay for influenza virus using an antibody which undergoes antigen-antibody reaction with influenza virus matrix 1 protein, or an antigen-binding fragment thereof, bringing a sample containing influenza virus into contact with a sample processing liquid containing a surfactant having at least one group selected from the group consisting of palmityl, stearyl, and oleyl, is provided.

摘要翻译： 提供了使用流感病毒M1蛋白作为抗原，在用于测量样本中的流感病毒的免疫测定中具有足够灵敏度的免疫测定的手段。 该流感病毒免疫测定法中的样品处理方法包括：在使用与流感病毒基质蛋白1发生抗原抗体反应的抗体或其抗原结合片段对流感病毒进行免疫测定的情况下，将含有流感病毒的样品 病毒与含有具有至少一个选自棕榈酰基，硬脂酰基和油酰基的基团的表面活性剂的样品处理液接触。

公开(公告)号：EP3029462A4

公开(公告)日：2017-03-22

申请号：EP14831189

申请日：2014-07-31

申请人： DENKA SEIKEN KK

发明人： KOHIYAMA RISA ,  ISHIKAWA OSAMU ,  MIYAZAWA TAKASHI

IPC分类号： G01N33/543 ,  G01N21/64 ,  G01N21/78 ,  G01N33/558

CPC分类号： G01N33/54313 ,  B01D15/3809 ,  G01N21/6428 ,  G01N33/545 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/0636

摘要： The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other. The immunochromatographic test piece is an immunochromatographic test piece comprising a membrane on which a capture substance being a ligand that bonds to a substance to be detected is immobilized, wherein insoluble carrier particles to which a ligand that bonds to the substance to be detected is bound are used and accumulated by being captured with the capture substance immobilized on the membrane, the membrane is irradiated with light to detect light emitted from a portion where the insoluble carrier particles are accumulated or light emitted from a portion surrounding and other than the portion where the insoluble carrier particles are accumulated, thereby measuring the substance to be detected, and a light-reflecting material is provided on a side of the membrane opposite to a side irradiated with light.

公开(公告)号：EP2905615A4

公开(公告)日：2016-05-11

申请号：EP13844402

申请日：2013-10-03

申请人： DENKA SEIKEN KK

发明人： MITSUMATA RYOTARO ,  IZUTANI NORIYUKI

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/569

CPC分类号： G01N33/56983 ,  G01N2333/11

摘要： Disclosed is a novel method for measuring haemagglutinin of an influenza virus, which can construct an assay system in a shorter period of time than a sandwich immunoassay method using two kinds of anti-haemagglutinin antibodies. The method for measuring haemagglutinin of an influenza virus is achieved by a sandwich immunoassay method comprising sandwiching the haemagglutinin between a lectin which binds to the haemagglutinin but does not bind to an antibody, and an anti-haemagglutinin antibody which undergoes antigen-antibody reaction with the haemagglutinin.

公开(公告)号：EP2682476A4

公开(公告)日：2015-06-17

申请号：EP12752957

申请日：2012-02-27

申请人： DENKA SEIKEN KK

发明人： HIGUCHI MAIKO ,  ITOH YASUKI

IPC分类号： C12Q1/44 ,  C12Q1/60 ,  G01N33/92

CPC分类号： G01N33/92 ,  C12Q1/60 ,  G01N2333/916

摘要： Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.

摘要翻译： 公开了一种用于定量测试样品中胆固醇HDL2，而不需要费力的操作的方法。 定量胆固醇的方法包括：使磷脂酶作用于HDL胆固醇量化。 游离缺失所以盘是一种方法，包括：比试验样品到反应体系外的高密度脂蛋白其它传递环的胆固醇的第一步骤; 和定量的高密度脂蛋白残留在反应体系中的高密度脂蛋白中胆固醇2的第二步骤; worin，通过对由上述方法中的第二步骤中，高密度脂蛋白胆固醇2测试样品中进行定量。

公开(公告)号：EP2426496A4

公开(公告)日：2013-03-13

申请号：EP10769753

申请日：2010-04-27

申请人： UNIV NIIGATA ,  JUNTENDO EDUCATIONAL FOUNDATION ,  DENKA SEIKEN KK

发明人： SAITO AKIHIKO ,  TOMINO YASUHIKO ,  ASANUMA KATSUHIKO ,  OGASAWARA SHINYA ,  KUROSAWA HIROYUKI ,  HIRAYAMA YOSHIAKI

IPC分类号： G01N33/53 ,  G01N33/68

CPC分类号： G01N33/6872 ,  G01N2800/347

公开(公告)号：EP2199404A4

公开(公告)日：2012-07-25

申请号：EP08830181

申请日：2008-09-11

申请人： DENKA SEIKEN KK

发明人： HIRAO YUHKO

IPC分类号： C12Q1/30 ,  C12Q1/60

CPC分类号： C12Q1/30

公开(公告)号：EP1739189A4

公开(公告)日：2009-07-08

申请号：EP05727370

申请日：2005-03-30

申请人： DENKA SEIKEN KK

发明人： MATSUMOTO KEIKO ,  MATSUI HIROSHI

IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  G01N33/92

CPC分类号： C12Q1/28 ,  C12Q1/60

摘要： Provided is a method of stabilizing a reagent that allows simultaneous quantification of LDL cholesterol and total cholesterol by a single measurement by suppressing spontaneous color development thereof. A method of quantification for cholesterol in low density lipoprotein and total cholesterol in a biological sample by the single measurement comprises a first step of treating lipoproteins other than low density lipoprotein in the biological sample to generate hydrogen peroxide and a second step of converting the hydrogen peroxide obtained in the first step to a quinone dye and treating remaining low density lipoprotein and converting generated hydrogen peroxide to the quinone dye, where the quinone dye is not formed in the first step, and cholesterol in low density lipoprotein and total cholesterol are quantified from the amount of the quinone dye formed in the second step by the single measurement.

公开(公告)号：EP2952896A4

公开(公告)日：2016-10-12

申请号：EP14745451

申请日：2014-01-31

申请人： DENKA SEIKEN KK

发明人： MIYAZAWA TAKASHI ,  SHINOHARA YUKI

IPC分类号： G01N33/543 ,  G01N33/53

CPC分类号： G01N33/5306 ,  G01N33/54393

摘要： The problem to be solved is accurately and specifically detecting a target substance by suppressing the action of an immunoassay inhibitory substance contained in a specimen derived from a biological mucous membrane. A false negative can be suppressed by treating a specimen with a specimen treatment solution containing a compound having two or more sulfate groups in immunoassay for analyzing a target substance in a specimen derived from a biological mucous membrane.

公开(公告)号：EP2837693A4

公开(公告)日：2015-12-30

申请号：EP13775869

申请日：2013-04-10

申请人： DENKA SEIKEN KK ,  UNIV HOKKAIDO NAT UNIV CORP

发明人： ITOH YASUKI ,  CHIBA HITOSHI

IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/44 ,  G01N33/92

CPC分类号： C12Q1/60 ,  G01N33/92 ,  G01N2333/775

摘要： Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.

公开(公告)号：EP2799870A4

公开(公告)日：2015-09-02

申请号：EP12861523

申请日：2012-12-28

申请人： DENKA SEIKEN KK

发明人： KANO MAYUMI ,  MIZUE HIROMI

IPC分类号： G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/5306

摘要： Provided is a means for improving measurement accuracy in an immunoassay using a dry plastic cell. A method for reducing adsorption of bubbles onto a cell side surface in an immunoassay, the method including carrying out a reaction and/or measurement in a presence of a surfactant, wherein the immunoassay comprises carrying out an antigen-antibody reaction in a dry plastic cell using an immunoassay reagent which immunologically reacts with a substance to be measured in a sample and carrying out an optical measurement of a resultant reacted product.