公开(公告)号：EP2250255A2

公开(公告)日：2010-11-17

申请号：EP09742964.1

申请日：2009-03-03

申请人： The Trustees of the University of Pennsylvania

发明人： ROY, Soumitra ,  WILSON, James, M. ,  VANDENBERGHE, Luc, H.

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K35/761 ,  A61K39/145 ,  A61K2039/5256 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10371 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16171

摘要： An adenovirus is disclosed, having a capsid comprising a hexon protein of SAdV-42.1 or an amino acid sequence at least about 99% identical thereto, or a hybrid hexon protein. The capsid encapsidates a heterologous nucleic acid molecule carrying a gene sequence operably linked to expression control sequences which direct transcription, translation, and/or expression in a host cell, and further encapsidates 5' and 3' adenovirus cis-elements necessary for replication and encapsidation.

公开(公告)号：EP2220241A2

公开(公告)日：2010-08-25

申请号：EP08857159.1

申请日：2008-11-24

申请人： The Trustees of the University of Pennsylvania

发明人： ROY, Soumitra ,  WILSON, James, M. ,  VANDENBERGHE, Luc, H.

IPC分类号： C12N15/861 ,  C12N7/04 ,  C07K14/075 ,  A61K39/00 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/525 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10361 ,  C12N2760/16122

摘要： A recombinant vector comprises simian adenovirus at least one SAdV-30 sequence and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-30 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

公开(公告)号：EP1409012B1

公开(公告)日：2009-02-11

申请号：EP02780874.0

申请日：2002-05-13

申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY ,  The Trustees of The University of Pennsylvania

发明人： ERTL, Hildegund, C., J. ,  WILSON, James, M.

IPC分类号： A61K39/295 ,  C12N15/861 ,  A61K39/235

CPC分类号： C12N15/86 ,  A61K39/00 ,  A61K39/235 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10362 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/20122 ,  C12N2760/20134 ,  C12N2830/002 ,  C12N2830/55 ,  Y02A50/489

摘要： A method of inducing a CD8+ T-cell response against a selected molecule by delivering the molecule via a recombinant simian adenovirus is provided. Also provided are methods of inducing interferon-alpha and interferon-beta by delivering a recombinant simian adenovirus to a subject. The methods of the invention are particularly well suited for prophylaxis and treatment of infections with human immunodeficiency virus and human papilloma virus, among others, and cancer therapy.

摘要翻译： 提供诱导针对所选分子通过经由重组猿猴腺病毒递送分子的CD8 + T细胞应答的方法。 如此提供的是通过提供一个重组猿猴腺病毒的受试者中诱导干扰素-α和干扰素-β的方法。 本发明的方法是特别适合用于预防和治疗人类免疫缺陷病毒和人乳头状瘤病毒，等等，和癌症治疗的感染。

公开(公告)号：EP2012822A2

公开(公告)日：2009-01-14

申请号：EP07835738.1

申请日：2007-04-27

申请人： The Trustees of the University of Pennsylvania

发明人： ROY, Soumitra ,  WILSON, James, M.

IPC分类号： A61K39/12

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/01 ,  C12N15/86 ,  C12N2710/10022 ,  C12N2710/10043 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2740/16134 ,  C12N2810/60 ,  C12N2810/6018 ,  C12N2810/6054 ,  C12N2810/85

摘要： The present invention provides a method of altering the specificity of an adenovirus vector. The method involves providing an adenovirus having a capsid with a modified adenovirus hexon protein. The modified adenovirus has a capsid comprising a hexon protein with a deletion in hypervariable region 1 and/or hypervariable region 4 of the hexon and an insert of an exogenous molecule therein.

摘要翻译： 本发明提供了改变腺病毒载体的特异性的方法。 该方法包括提供具有修饰的腺病毒六邻体蛋白的衣壳的腺病毒。 修饰的腺病毒具有包含在六倍体的高变区1和/或高变区4中的缺失的六邻体蛋白和其中的外源性分子的插入物的衣壳。

公开(公告)号：EP0932418B1

公开(公告)日：2007-12-05

申请号：EP97943290.3

申请日：1997-09-04

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： WILSON, James, M. ,  FISHER, Krishna, J.

IPC分类号： A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2750/14143

摘要： A method of prolonging gene expression by reducing immune response to a recombinant adeno-associated virus (AAV) bearing a desired gene administered into the muscle of a mammal is described.

公开(公告)号：EP1743028A2

公开(公告)日：2007-01-17

申请号：EP05739761.4

申请日：2005-04-27

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： GAO, Guangping ,  WILSON, James, M. ,  ZHOU, Xiangyang

IPC分类号： C12N15/86 ,  C12Q1/68

CPC分类号： C12N15/85 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2800/101 ,  C12N2800/107 ,  C12N2800/70 ,  C12N2840/20

摘要： A system for generating viral vectors carrying two distinct expression cassettes is provided. The system utilizes a unique polyvalent transfer vector that permits efficient detection and selection of inserted expression cassettes.

公开(公告)号：EP1636370A2

公开(公告)日：2006-03-22

申请号：EP04753443.3

申请日：2004-06-15

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： ROY, Soumitra ,  WILSON, James, M.

IPC分类号： C12N15/86 ,  C12N5/10 ,  C12N7/01 ,  C07K14/075 ,  A61K39/235 ,  A61K48/00 ,  C12N15/34

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2710/10351 ,  C12N2760/14134 ,  C12N2810/6018

摘要： A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which grow efficiently in the desired cell line. At a minimum, the left terminus spans the (5') inverted terminal repeat, the left terminus spans the E4 region and the (3') inverted terminal repeat. The resulting chimeric adenovirus contains the internal regions spanning the genes encoding the penton, hexon and fiber from the serotype which does not grow efficiently in the desired cell. Also provided are vectors constructed from novel simian adenovirus sequences and proteins, host cells containing same, and uses thereof.

公开(公告)号：EP0797678B1

公开(公告)日：2001-03-21

申请号：EP95942840.0

申请日：1995-10-27

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： WILSON, James, M. ,  KELLEY, William, M. ,  FISHER, Krishna, J.

IPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/47 ,  C07K14/775

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4712 ,  C07K14/705 ,  C12N15/87 ,  C12N2710/10344 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2810/40 ,  C12N2830/008

摘要： The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.

摘要翻译： 新型重组杂合病毒（I）包含：（a）腺病毒的5'末端重复序列（ITR）和5'腺病毒包装/增强子结构域的DNA序列; （b）5'腺相关病毒（AAV）ITR序列的DNA序列; （c）编码与体内或体外指导蛋白质在靶细胞中表达的调节序列可操作地连接的所选蛋白质的基因; （d）3'AAV ITR序列的DNA序列; （e）3'腺病毒ITR序列的DNA序列; 其中病毒是复制缺陷的，并且被提供有足够部分的腺病毒基因组以允许靶细胞的感染。

公开(公告)号：EP0960191A1

公开(公告)日：1999-12-01

申请号：EP97938455.0

申请日：1997-08-20

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  MAGAININ PHARMACEUTICALS INC.

发明人： WILSON, James, M. ,  GOLDMAN, Mitchell ,  BALS, Robert ,  STOLZENBERG, Ethan, D. ,  ANDERSON, Mark ,  ZASLOFF, Michael ,  KARI, Prasad

IPC分类号： C12N15 ,  A61K38 ,  A61K48 ,  A61P11 ,  A61P17 ,  A61P31 ,  C07K14 ,  C12N5

CPC分类号： C07K14/4723 ,  A61K38/00

摘要： The invention relates to mammalian beta defensin and methods of use thereof for treatment of microbial infection.

公开(公告)号：EP0950111A1

公开(公告)日：1999-10-20

申请号：EP97939821.0

申请日：1997-09-04

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： WILSON, James, M. ,  PHANEUF, Daniel

IPC分类号： C12N15 ,  C07K14 ,  C12N5 ,  C12N7 ,  C12N9 ,  A61K48

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N9/00 ,  C12N2710/10343 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2810/40 ,  C12N2830/003 ,  C12N2830/42

摘要： Methods for efficient production of recombinant AAV are described. In one aspect, three vectors are introduced into a host cell. A first vector directs expression of cre recombinase, a second vector contains a promoter, a spacer sequence flanked by loxP sites and rep/cap, and a third vector contains a minigene containing a transgene and regulatory sequences flanked by AAV ITRs. In another aspect, the host cell stably or inducibly expresses cre recombinase and two vectors carrying the other elements of the system are introduced into the host cell.