公开(公告)号：EP2099485B1

公开(公告)日：2018-03-21

申请号：EP07844849.5

申请日：2007-11-02

申请人： AlphaVax, Inc.

发明人： DEPAZ, Roberto A. ,  TALARICO, Todd L.

IPC分类号： A61K39/12 ,  A61K9/19 ,  C12N15/86 ,  C12N7/00 ,  A61K39/00

CPC分类号： C12N7/00 ,  A61K9/19 ,  A61K39/12 ,  A61K2039/525 ,  A61K2039/5256 ,  C12N2770/36134 ,  C12N2770/36151

摘要： Disclosed are methods for preparing dried (preferably lyophilized) preparations comprising a population of alphaviruses or alphavirus replicon particles, a sugar or polyol, a surfactant and a salt and preparations made by said methods, both in the dried form but also as liquids prior to drying or after reconstituting dried preparations. These preparations may further comprise a plasticizer and/or a bulking agent. These preparations are readily reconstituted, with little or no loss in infectivity of the viruses or replicon particles.

公开(公告)号：EP2290054B1

公开(公告)日：2017-03-29

申请号：EP10011537.7

申请日：2003-12-12

申请人： Alphavax, Inc.

发明人： Smith, Jonathan F. ,  Kamrud, Kurt ,  Dryga, Sergey ,  Alterson, Harold ,  Rayner, Jon ,  Alterson, Kim ,  Maughan, Maureen F.

IPC分类号： C12N7/02 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/36143 ,  C12N2770/36151 ,  C12N2770/36162

公开(公告)号：EP2416662A1

公开(公告)日：2012-02-15

申请号：EP10762452.0

申请日：2010-04-08

申请人： Alphavax, Inc. ,  Sloan-Kettering Institute for Cancer Research

发明人： WOLCHOK, Jedd D. ,  AVOGADRI, Francesca ,  OLMSTED, Robert A. ,  MAUGHAN, Maureen

IPC分类号： A01N63/00 ,  A61K39/12

CPC分类号： A61K39/0011 ,  A61K2039/5254 ,  A61K2039/5256

摘要： The immune response to melanoma cells and tumors can be induced or significantly increased by administering to a subject a pharmaceutical composition comprising alphavirus particles, especially Venezuelan equine encephalitis virus replicon particles, which express the melanoma antigen dopachrome tautomerase (DCT, TRP2) in cells of the subject, with the result of tumor regression and /or inhibition of metastasis of a melanoma subject, or a decreased risk of the occurrence or recurrence of melanoma and/or decreased severity of melanoma in a subject not suffering from melanoma at the time of administration. The pharmaceutical composition described herein can be used in conjunction with other therapeutic agents, it can be administered on more than one occasion and it can be combined with administrations of other compositions such as protein or other immunogenic compositions, and/or adjuvants, with beneficial effects to the human or animal subject to which it has been administered.

公开(公告)号：EP1432791B1

公开(公告)日：2009-07-22

申请号：EP02763613.3

申请日：2002-09-06

申请人： Alphavax, Inc.

发明人： SMITH, Jonathan, F. ,  KAMRUD, Kurt, I. ,  RAYNER, Jonathan, O. ,  DRYGA, Sergey, A. ,  CALEY, Ian, J.

IPC分类号： C12N7/02 ,  C12N15/86 ,  C12N5/10

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162 ,  C12N2840/203 ,  C12N2840/206

摘要： The present invention provides compositions useful in and methods for producing populations of infectious, replication-defective alphavirus replicon particles that contain no replication-competent alphavirus particles, as determined by passage on cells in culture. The compositions include helper and replicon nucleic acid molecules that can further reduce the predicted frequency for formation of replication-competent virus and can optimize manufacturing strategies and costs.

公开(公告)号：EP1590451A2

公开(公告)日：2005-11-02

申请号：EP03813433.4

申请日：2003-12-12

申请人： Alphavax, Inc. ,  Smith, Jonathan F. ,  Kamrud, Kurt ,  Dryga, Sergey ,  Alterson, Harold ,  Rayner, Jon ,  Butler, Kim ,  Maughan, Maureen F.

发明人： SMITH, Jonathan, F. ,  KAMRUD, Kurt ,  DRYGA, Sergey ,  ALTERSON, Harold ,  RAYNER, Jon ,  ALTERSON, Kim ,  MAUGHAN, Maureen, F.

IPC分类号： C12N7/02 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/36143 ,  C12N2770/36151 ,  C12N2770/36162

摘要： Provided herein are methods for producing alphavirus replicon particles in high yield; replicon RNAs are electroporated into permissive cells, where the cells are at a relatively high density, together with at least one helper nucleic acid providing the necessary functions for packaging. After a growth period in appropriate medium, alphavirus replicon particles are harvested from the surfaces of the cells in which they were produced using a salt wash in which the salt concentration is from about 0.2 to about 5 M sodium chloride, calcium chloride, magnesium chloride, potassium chloride, ammonium acetate, ammonium bicarbonate, among others. After dilution, if necessary, the particles can be purified by a suitable chromatographic technique.

公开(公告)号：EP1585812A2

公开(公告)日：2005-10-19

申请号：EP03813432.6

申请日：2003-12-12

申请人： Alphavax, Inc.

发明人： SMITH, Jonathan, F. ,  KAMRUD, Kurt ,  DRYGA, Sergey ,  CALEY, Ian

IPC分类号： C12N7/00 ,  C12N15/63 ,  C12N5/06 ,  C12N5/10 ,  C12N15/86 ,  C07H21/02 ,  A01N63/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/70 ,  C12N15/86 ,  C12N2740/16222 ,  C12N2770/36134 ,  C12N2770/36143 ,  C12N2770/36152

摘要： Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

摘要翻译： 病毒复制子选择的核酸表达文库可用于分析与寄生虫，病原体或瘤形成有关的多种抗原或用于制备用于产生对寄生虫病原体或瘤形成特异性的免疫应答的免疫原性组合物。 代表核酸表达文库的甲病毒属复制子颗粒是优选的。 核酸文库可以是随机文库，或者可以在选择步骤之后制备，例如在克隆入复制子载体之前通过差异杂交。

公开(公告)号：EP2066346B1

公开(公告)日：2019-07-03

申请号：EP07872297.2

申请日：2007-09-12

申请人： AlphaVax, Inc.

发明人： SMITH, Jonathan F. ,  HUBBY, Bolyn ,  BERGLUND, Peter ,  COPP, Laura ,  ELLIS, Whitney

IPC分类号： A61K39/145

公开(公告)号：EP2061508B1

公开(公告)日：2014-10-15

申请号：EP07842383.7

申请日：2007-09-12

申请人： Alphavax, Inc.

发明人： SMITH, Jonathan, F. ,  HUBBY, Bolyn ,  COPP, Laura

IPC分类号： A61K39/39 ,  A61K39/145 ,  C12N15/86 ,  C12N7/04

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/70 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/16134 ,  C12N2770/36143

公开(公告)号：EP1608762B1

公开(公告)日：2014-01-08

申请号：EP04757890.1

申请日：2004-03-19

申请人： Alphavax, Inc.

发明人： SMITH, Jonathan, F. ,  KAMRUD, Kurt ,  RAYNER, Jon, O.

IPC分类号： C12N15/86 ,  A61K35/76 ,  C07K14/18

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/53 ,  C12N7/00 ,  C12N15/86 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2770/36143 ,  C12N2770/36161 ,  C12N2770/36162 ,  C12N2820/60 ,  C12N2840/203

公开(公告)号：EP2290054A1

公开(公告)日：2011-03-02

申请号：EP10011537.7

申请日：2003-12-12

申请人： Alphavax, Inc.

发明人： Smith, Jonathan F. ,  Kamrud, Kurt ,  Dryga, Sergey ,  Alterson, Harold ,  Rayner, Jon ,  Alterson, Kim ,  Maughan, Maureen F.

IPC分类号： C12N7/02 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/36143 ,  C12N2770/36151 ,  C12N2770/36162

摘要： Provided herein are methods for producing alphavirus replicon particles in high yield; replicon RNAs are electroporated into permissive cells, where the cells are at a relatively high density, together with at least one helper nucleic acid providing the necessary functions for packaging. After a growth period in appropriate medium, alphavirus replicon particles are harvested from the surfaces of the cells in which they were produced using a salt wash in which the salt concentration is from about 0.2 to about 5 M sodium chloride, calcium chloride, magnesium chloride, potassium chloride, ammonium acetate, ammonium bicarbonate, among others. After dilution, if necessary, the particles can be purified by a suitable chromatographic technique.