公开(公告)号：EP2260130A4

公开(公告)日：2012-04-04

申请号：EP09727374

申请日：2009-04-03

申请人： ALLOPARTIS BIOTECHNOLOGIES INC ,  SCRIPPS RESEARCH INST

发明人： BLAZEJ ROBERT G ,  PAEGEL BRIAN M

IPC分类号： C40B30/00

CPC分类号： C12N15/1075

公开(公告)号：EP2260130B1

公开(公告)日：2013-10-23

申请号：EP09727374.2

申请日：2009-04-03

申请人： Allopartis Biotechnologies, Inc. ,  The Scripps Research Institute

发明人： BLAZEJ, Robert, G. ,  PAEGEL, Brian M.

IPC分类号： C40B30/00

CPC分类号： C12N15/1075

公开(公告)号：EP2260130A2

公开(公告)日：2010-12-15

申请号：EP09727374.2

申请日：2009-04-03

申请人： Allopartis Biotechnologies, Inc. ,  The Scripps Research Institute

发明人： BLAZEJ, Robert, G. ,  PAEGEL, Brian, M.

IPC分类号： C40B30/00

CPC分类号： C12N15/1075

摘要： Described are methods and means for enhancing enzyme activity toward insoluble substrates. This is achieved by means of in vitro compartmentalization in which an insoluble microparticle functions both as the enzyme substrate and as a structure for negative selection. Enhanced enzymes expressed from a microparticle-linked polynucleotide library preferentially degrade the microparticle releasing specific gene variants into solution. Gene variants encoding less active enzyme variants remain linked to the microparticle and may be removed through centrifugation, thus enriching the polynucleotide library for more active enzyme variants. These methods may be used to enhance cellulase and ligninase activity toward insoluble cellulosic biomass.