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13 条记录 (耗时 0.018 秒)

    Alpha-1-antitrypsin composition
    6.
    发明授权
    Alpha-1-antitrypsin composition 有权
    α-1-抗胰蛋白酶Zusammensetzung

    公开(公告)号：EP2295126B1

    公开(公告)日：2015-12-16

    申请号：EP10182334.2

    申请日：2003-12-19

    申请人： CSL Behring LLC

    发明人： Cook, Paul I. ,  Fowler, Scott A ,  Kee, Scott M. ,  Smith, James R. ,  Weber, David ,  Kling, Robert

    IPC分类号： C07K14/81

    CPC分类号： A61K38/57 ,  A61K9/19 ,  B01D15/327 ,  B01D15/363 ,  C07K14/8125 ,  G01N30/461

    摘要： A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

    摘要翻译： 提供了从含有AAT的蛋白质混合物如Cohn馏分IV沉淀物中纯化α-1-抗胰蛋白酶（AAT）的流线型方法。 在本发明的方法中，通过用不影响AAT的还原剂如二硫醇裂解二硫键，污染蛋白质不稳定。 然后将不稳定的蛋白质优先吸附在固体蛋白质吸附材料上，而不加入作为沉淀剂的盐。 固体吸附剂与溶液的分离留下直接适用于色谱纯化的纯化的AAT溶液，而不需像现有技术方法那样进行广泛的脱盐。 还描述了一种以商业规模提供高产量的药物级AAT的方法。

    A METHOD OF PURIFYING PROTEINS
    7.
    发明公开
    A METHOD OF PURIFYING PROTEINS 审中-公开
    一种纯化蛋白质的方法

    公开(公告)号：EP2904005A1

    公开(公告)日：2015-08-12

    申请号：EP13777203.4

    申请日：2013-10-01

    申请人： CSL Behring LLC

    发明人： BRINKMAN, Nathan

    IPC分类号： C07K14/79 ,  C07K1/14 ,  C07K1/16 ,  C07K1/30 ,  C07K1/36 ,  C07K14/47

    CPC分类号： C07K14/805 ,  C07K14/4717

    摘要： The present invention relates generally to a method of purifying proteins. More specifically, the present inventions relates to a method of purifying haptoglobin and hemopexin from the same starting material, and uses thereof.

    摘要翻译： 本发明一般涉及纯化蛋白质的方法。 更具体地说，本发明涉及从相同的起始材料中纯化触珠蛋白和血红素结合蛋白的方法及其用途。

    Method for purification of alpha-1-antitrypsin
    8.
    发明公开
    Method for purification of alpha-1-antitrypsin 有权
    α-1抗胰蛋白酶的组合物

    公开(公告)号：EP2295126A1

    公开(公告)日：2011-03-16

    申请号：EP10182334.2

    申请日：2003-12-19

    申请人： CSL Behring LLC

    发明人： Cook, Paul I. ,  Fowler, Scott A ,  Kee, Scott M. ,  Smith, James R. ,  Weber, David ,  Kling, Robert

    IPC分类号： B01D15/08 ,  C07K14/81

    CPC分类号： A61K38/57 ,  A61K9/19 ,  B01D15/327 ,  B01D15/363 ,  C07K14/8125 ,  G01N30/461

    摘要： A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.