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公开(公告)号：EP3351644B1
公开(公告)日：2020-01-29
申请号：EP18161068.4
申请日：2013-11-28
发明人： OST, Toby
IPC分类号： C12Q1/6827
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公开(公告)号：EP4034676A1
公开(公告)日：2022-08-03
申请号：EP21755018.5
申请日：2021-07-29
发明人： BALASUBRAMANIAN, Shankar , FULLGRABE, Jens , GOSAL, Walraj Singh , HOLBROOK, Joanna Dawn , LIU, Sidong , MORLEY, David , NENTWICH, Oliver , OST, Tobias , STEWARD, Michael , VILELLA, Albert , WALKER, Nicholas James , YU, Shirong , BIGNELL, Helen Rachel , SAN-BENTO, Rita Santo
IPC分类号： C12Q1/6869
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公开(公告)号：EP4083231A1
公开(公告)日：2022-11-02
申请号：EP22170290.5
申请日：2021-07-29
发明人： BALASUBRAMANIAN, Shankar , FULLGRABE, Jens , GOSAL, Walraj Singh , HOLBROOK, Joanna Dawn , LIU, Sidong , MORLEY, David , NENTWICH, Oliver , OST, Tobias , STEWARD, Michael , VILELLA, Albert , WALKER, Nicholas James , YU, Shirong , BIGNELL, Helen Rachel , SAN-BENTO, Rita Santo
IPC分类号： C12Q1/6869
摘要： Provided herein are methods, systems, and compositions for determining a base in a polynucleotide. In various aspects, the methods, systems, and compositions presented herein are useful for performing 4-base, 5-base, or 6-base sequencing of polynucleotide molecules, for example, from liquid biopsy samples or wherein the base is a low frequency mutation.
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公开(公告)号：EP3625365A2
公开(公告)日：2020-03-25
申请号：EP18749488.5
申请日：2018-05-15
发明人： STEWARD, Michael , OST, Tobias , YU, Shirong , BIGNELL, Helen
IPC分类号： C12Q1/6858
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公开(公告)号：EP3110969A1
公开(公告)日：2017-01-04
申请号：EP15707727.2
申请日：2015-02-24
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2521/131 , C12Q2523/107 , C12Q2523/125 , C12Q2525/121 , C12Q2525/301 , C12Q2535/122 , C12Q2525/186 , C12Q2525/191 , C12Q2543/101 , C12Q2565/1015
摘要： This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.
摘要翻译： 本发明涉及用于分析的核酸样品的制备。 本发明对于单链样品可能特别有用。 本发明的实施方案涉及在制备核酸测序文库中使用模板无关聚合酶连接双链或发夹寡核苷酸。
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公开(公告)号：EP3351644A1
公开(公告)日：2018-07-25
申请号：EP18161068.4
申请日：2013-11-28
发明人： OST, Toby
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2600/154 , C12Q2523/115 , C12Q2523/125
摘要： This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. Methods, reagents and kits are provided.
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公开(公告)号：EP3174996A1
公开(公告)日：2017-06-07
申请号：EP15744329.2
申请日：2015-07-28
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , C12Q1/6876 , C12Q2600/154 , C12Q2523/125 , C12Q2525/117 , C12Q2525/191 , C12Q2563/185
摘要： The inventors have developed a set of molecular tag adaptors allowing the history of a sample to be followed. The adaptors have a region having more than one type of cytosine base.
摘要翻译： 本发明人开发了一组允许遵循样品历史的分子标签适配器。 衔接子具有一个以上一种胞嘧啶碱基的区域。
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公开(公告)号：EP2925883A1
公开(公告)日：2015-10-07
申请号：EP13801511.0
申请日：2013-11-28
发明人： OST, Toby
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2600/154 , C12Q2523/115 , C12Q2523/125
摘要： This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. A first portion of the population is oxidised with a metal (VI) oxo complex and then the first portion and a second portion of said population are both treated with bisulfite. The residues in the first and second portions that correspond to a cytosine residue in the sample nucleotide sequence are identified following treatment and the identities of these residues are used to determine the modification of the cytosine residue in the sample nucleotide sequence. Methods, reagents and kits are provided.
摘要翻译： 本发明涉及金属（Ⅵ）氧代络合物催化多核苷酸中5mCc残基至5fC残基的选择性氧化的用途。 这可用于鉴定包含样品核苷酸序列的多核苷酸群体中修饰的胞嘧啶残基。 群体的第一部分用金属（VI）氧化络合物氧化，然后所述群体的第一部分和第二部分都用亚硫酸氢盐处理。 在处理后鉴定与样品核苷酸序列中的胞嘧啶残基相对应的第一部分和第二部分中的残基，并且使用这些残基的身份来确定样品核苷酸序列中胞嘧啶残基的修饰。 提供方法，试剂和试剂盒。
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