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公开(公告)号：EP1257823B1
公开(公告)日：2004-02-04
申请号：EP01919280.6
申请日：2001-02-14
申请人： Immundiagnostik AG , Ganzimmun Ag
IPC分类号： G01N33/569
CPC分类号： G01N33/56922 , G01N33/54306 , G01N33/56911 , G01N33/6854
摘要： The invention relates to a method for detecting pathogenic organisms, especially Helicobacter pylori and H. heilmanii , in fecal, salivary and secretion specimen by a double antibody sandwich assay. The inventive method is characterized by dissolving or dispersing the specimen together with a pathogenic antigen in a buffer solution and contacting the buffer solution with a solid phase to which at least two primary antibodies are bound, one of which specifically binds to the pathogenic antigen and the other to human immunoglobulin A; washing the solid phase of non-specifically bound proteins and contacting the solid phase with a secondary antibody that specifically binds to the pathogenic antigen; and determining the amount of specifically bound secondary antibody.
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公开(公告)号：EP1257823A2
公开(公告)日：2002-11-20
申请号：EP01919280.6
申请日：2001-02-14
申请人： Immundiagnostik AG , Ganzimmun Ag
IPC分类号： G01N33/569
CPC分类号： G01N33/56922 , G01N33/54306 , G01N33/56911 , G01N33/6854
摘要： The invention relates to a method for detecting pathogenic organisms, especially Helicobacter pylori and H. heilmanii, in fecal, salivary and secretion specimen by a double antibody sandwich assay. The inventive method is characterized by dissolving or dispersing the specimen together with a pathogenic antigen in a buffer solution and contacting the buffer solution with a solid phase to which at least two primary antibodies are bound, one of which specifically binds to the pathogenic antigen and the other to human immunoglobulin A; washing the solid phase of non-specifically bound proteins and contacting the solid phase with a secondary antibody that specifically binds to the pathogenic antigen; and determining the amount of specifically bound secondary antibody.
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