公开(公告)号：EP2970668A2

公开(公告)日：2016-01-20

申请号：EP14762805.1

申请日：2014-03-17

申请人： Bio-rad Laboratories, Inc. ,  Chia, Erin, R. ,  Hiddessen, Amy, L. ,  Hindson, Benjamin, J. ,  Lowe, Adam ,  Han, Chunxiao

发明人： CHIA, Erin, R. ,  HIDDESSEN, Amy, L. ,  HINDSON, Benjamin, J. ,  LOWE, Adam ,  HAN, Chunxiao

IPC分类号： C08L83/06

CPC分类号： C07F7/184 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

公开(公告)号：EP2970668A4

公开(公告)日：2016-08-10

申请号：EP14762805

申请日：2014-03-17

申请人： BIO RAD LABORATORIES ,  CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

发明人： CHIA ERIN R ,  HIDDESSEN AMY L ,  HINDSON BENJAMIN J ,  LOWE ADAM ,  HAN CHUNXIAO

IPC分类号： G01N33/50 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14

CPC分类号： C07F7/184 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

摘要翻译： 用于制备和使用包含硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 含水液滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于这些信号来确定分析物的特征，例如分析物水平或活性。