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1.发明公开METHOD AND DEVICE FOR DETERMINING CONCENTRATION, CROSSTALK AND DISPLACEMENT FLUORESCENCE CROSS CORRELATION SPECTROSCOPY 审中-公开确定浓度，过渡和移位荧光互相关光谱的方法和装置
公开(公告)号：EP2707700A4
公开(公告)日：2014-10-29
申请号：EP12785119
申请日：2012-05-11
CPC分类号： G01N21/6428 , C12Q1/6818 , G01N21/6408 , G01N33/542 , G01N33/581 , G01N33/582 , G01N2021/6417 , G01N2021/6432 , G01N2021/6441
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2.发明公开METHOD AND DEVICE FOR DETERMINING CONCENTRATION, CROSSTALK AND DISPLACEMENT FLUORESCENCE CROSS CORRELATION SPECTROSCOPY 审中-公开方法和设备，用来确定的浓度下，以约口语和转变荧光交叉相关光谱法
公开(公告)号：EP2707700A1
公开(公告)日：2014-03-19
申请号：EP12785119.4
申请日：2012-05-11
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , C12Q1/6818 , G01N21/6408 , G01N33/542 , G01N33/581 , G01N33/582 , G01N2021/6417 , G01N2021/6432 , G01N2021/6441
摘要： The present invention provides a FCCS method for determining the concentration and/or the diffusion coefficient of at least a first labeled species, a second labeled species and/or a complex between said first and second labeled species, in a system, wherein the method comprises the steps of determining a cross-talk parameter K, wherein K is the ratio between the brightness of the first labeled species and the second labeled species at the centre of each focus, as detected for both species in the channel for detecting the second labeled species; using the cross talk parameter K for determining a displacement parameter r
o and using K, r
o , or both K and r
o for determining the concentration and/or the diffusion coefficient of said first and/or a second labeled species and/or a complex between said first and second labeled species.
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