METHODS FOR MEASURING TELOMERE LENGTH
    2.
    发明公开
    METHODS FOR MEASURING TELOMERE LENGTH 失效
    程序为长度测量端粒

    公开(公告)号:EP0774014A1

    公开(公告)日:1997-05-21

    申请号:EP96921515.0

    申请日:1996-06-06

    IPC分类号: G01N27 C12N15 C12Q1

    摘要: Methods and compositions for the measurement of telomere length have application in medical diagnostic, prognostic, and therapeutic procedures. The methods for measuring telomere length include primer extension-based methods and probe-based methods. The primer extension methods involve elongation of telomeric, linker, and/or subtelomeric based primers under conditions such that the telomere serves as a template for primer extension and that the resultant primer extension products can be compared to standards of known length to provide a measure of telomere length. The probe based methods allow for telomere length measurements using DNA from lysed or whole cells and involve hydridizing an excess of probe to all telomeric repeat sequences in the telomere, measuring the amount of bound probe, and correlating the amount of bound probe measured with telomere length.

    METHODS FOR MEASURING TELOMERE LENGTH
    3.
    发明公开
    METHODS FOR MEASURING TELOMERE LENGTH 失效
    程序为长度测量端粒

    公开(公告)号:EP0774014A4

    公开(公告)日:2002-01-02

    申请号:EP96921515

    申请日:1996-06-06

    IPC分类号: G01N27/447 C12N15/09 C12Q1/68

    摘要: Methods and compositions for the measurement of telomere length have application in medical diagnostic, prognostic, and therapeutic procedures. The methods for measuring telomere length include primer extension-based methods and probe-based methods. The primer extension methods involve elongation of telomeric, linker, and/or subtelomeric based primers under conditions such that the telomere serves as a template for primer extension and that the resultant primer extension products can be compared to standards of known length to provide a measure of telomere length. The probe based methods allow for telomere length measurements using DNA from lysed or whole cells and involve hydridizing an excess of probe to all telomeric repeat sequences in the telomere, measuring the amount of bound probe, and correlating the amount of bound probe measured with telomere length.