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公开(公告)号：EP4393591A1
公开(公告)日：2024-07-03
申请号：EP22860635.6
申请日：2022-08-26
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： MA, Liang
IPC: B01L3/00
Abstract: A control device for micro-droplet preparation, the control device including a micro-droplet preparation unit, wherein the micro-droplet preparation unit comprises an asymmetric vibrating micro-droplet generation mechanism (1) and a first dynamic positioning assembly (200); the first dynamic positioning assembly (200) is fixedly connected to the asymmetric vibrating micro-droplet generation mechanism (1); the first dynamic positioning assembly (200) is configured to precisely position the asymmetric vibrating micro-droplet generation mechanism (1); and the asymmetric vibrating micro-droplet generation mechanism (1) is configured to generate micro-droplets through asymmetric reciprocating motion. The use of the control device enables the micro-droplets having a uniform size and a controllable volume to be prepared only by regulating a flow rate of a liquid sample and a vibration frequency of a sample injector.
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公开(公告)号：EP3258273A1
公开(公告)日：2017-12-20
申请号：EP17175313.0
申请日：2017-06-09
Applicant: Peking Union Medical College Hospital, Chinese Academy of Medical Sciences , Sichuan Maccura Biotechnology Co., Ltd.
Inventor： QIU, Ling , LONG, Tengxiang , GUO, Xiuzhi , WU, Changying
CPC classification number: G01N33/70
Abstract: The present invention relates to a kit and a method for detecting creatinine. A creatinine assay kit comprises the first reagent set and the second reagent set; the first reagent set contains N-(2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline or its salts, N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline or its salts, and combination thereof, and the second reagent set contains 4-aminoantipyrine. The kit and method of the present invention can decrease interference caused by calcium dobesilate and/ or etamsylate in sample when quantifying creatinine amount in a biological sample.
Abstract translation: 本发明涉及用于检测肌酸酐的试剂盒和方法。 肌酸酐测定试剂盒包含第一试剂组和第二试剂组; 第一试剂组含有N-（2-羟基-3-磺丙基）-3,5-二甲氧基苯胺或其盐，N-乙基-N-（2-羟基-3-磺丙基）-3,5-二甲氧基苯胺或其盐 及其组合，并且第二试剂组含有4-氨基安替比林。 当定量生物样品中的肌酸酐量时，本发明的试剂盒和方法可以减少由样品中的羟苯磺酸钙和/或乙磺酸钙造成的干扰。
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公开(公告)号：EP4458986A1
公开(公告)日：2024-11-06
申请号：EP22914834.1
申请日：2022-12-27
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , HU, Wan er , ZHAN, Haomiao
Abstract: Provided in the present invention is a method for detecting and differentiating multiple different targets in a single-tube PCR reaction by using single-tube ultra-multiplex PCR. The method can avoid false positive signals generated by a primer dimer when multiple specific primers are present. Further provided in the present invention are a primer and probe for the single-tube super-multiplex PCR, a reaction system, and a multiple-target detection kit containing the primer, the probe and the reaction system.
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公开(公告)号：EP4458991A1
公开(公告)日：2024-11-06
申请号：EP22914812.7
申请日：2022-12-27
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , HU, Waner , ZHAN, Haomiao , TANG, Fang
IPC: C12Q1/70
Abstract: Disclosed are a primer probe for PCR detection, a primer probe set, and an application thereof. The primer probe and the primer probe set can simultaneously perform analysis in two dimensions of a fluorescent channel and a melting temperature in a single tube reaction. That is, by using a same fluorescent channel, different targets may be detected by means of melting temperature characteristics; or when different fluorescent channels are used, a target type detection of a product of the number of fluorescent channels and the melting temperature characteristic may be achieved. Moreover, a method for performing multiplex PCR detection by using the primer probe has the advantages of being low in fluorescence background, adjustable in melting temperature, good in inclusiveness, and low in cost, achieving single tube reaction, and being simple and convenient to operate, not easy to cause pollution, high in sensitivity, and wide in applicable range.
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Patent Agency Ranking
公开(公告)号：EP4219737A1
公开(公告)日：2023-08-02
申请号：EP20954447.7
申请日：2020-09-23
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , HUANG, Qiuping , HE, Huihuang
IPC: C12Q1/68
Abstract: A combination, a method and a kit for detecting nucleic acids. The combination comprises: an upstream primer, wherein a target sequence binding region is provided at the 3'-end thereof, and the 3'-end of the target sequence binding region is capable of being complementary to a specific detection site of a target sequence; a downstream primer, wherein a target sequence binding region is provided at the 3'-end thereof, and the 3'-end of the target sequence binding region is capable of being complementary to the same specific detection site; and a signal oligonucleotide, wherein the signal oligonucleotide is modified with a first detection group and a second detection group, and the first detection group and the second detection group generate a change in signal by means of a change in distance.
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公开(公告)号：EP4458985A1
公开(公告)日：2024-11-06
申请号：EP22914814.3
申请日：2022-12-27
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , HU, Waner , WANG, Haixin , HUANG, Qiuping
Abstract: The present invention relates to the field of molecular biology, in particular to a method for detecting multiple target nucleic acids in a single sample container. The present invention particularly relates to a method for multiplex detection based on digital PCR. The method comprises: mixing a primer-probe composition, a sample to be detected and an amplification reagent to obtain a reaction system; placing the reaction system in a condition that allows a nucleic acid polymerase to perform hybridization and extension reaction, so as to obtain a reaction product; and performing signal acquisition on the reaction product. The detection method of the present invention can realize detection of multiple target nucleic acids, is easy to operate, and can save time.
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公开(公告)号：EP4458984A1
公开(公告)日：2024-11-06
申请号：EP22914805.1
申请日：2022-12-27
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , HU, Waner , WANG, Haixin , HUANG, Qiuping , GE, Zhiqi
Abstract: Provided is a method for detecting a target nucleic acid, in particular a method for detecting a single or multiple target nucleic acid on digital PCR by using a universal probe. The detection method can achieve single-tube ultra-multiple detection, and has the advantages of a low fluorescence background, an adjustable melting temperature, good inclusiveness, low costs, simple and convenient operation, not being prone to causing pollution, high sensitivity, a wide application range and the like.
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公开(公告)号：EP4219757A1
公开(公告)日：2023-08-02
申请号：EP20954964.1
申请日：2020-11-13
Applicant: Maccura Biotechnology Co., Ltd.
Inventor： ZHAO, Yuhang , ZHAN, Haomiao , HUANG, Mei
Abstract: Disclosed in the present invention are a multiplex nucleic acid detection method, a composition and a kit. The method comprises a multiplex nucleic acid detection method, which comprises the following steps: mixing a composition containing a first primer set and a first probe with a sample from a subject; amplifying a target sequence that may be present in the sample; performing a melting curve analysis to an amplified product in a corresponding detection channel; and judging whether one or more targets are present, according to a result of the melting curve analysis.
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公开(公告)号：EP3258273B1
公开(公告)日：2020-05-06
申请号：EP17175313.0
申请日：2017-06-09
Applicant: Peking Union Medical College Hospital, Chinese Academy of Medical Sciences , Sichuan Maccura Biotechnology Co., Ltd.
Inventor： QIU, Ling , LONG, Tengxiang , GUO, Xiuzhi , WU, Changying
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