公开(公告)号：EP3048170A4

公开(公告)日：2017-05-03

申请号：EP14846493

申请日：2014-09-19

申请人： NAT INST OF BIOMEDICAL INNOVATION HEALTH AND NUTRITION ,  DAIICHI SANKYO CO LTD

发明人： ISHII KEN ,  KOBIYAMA KOUJI ,  AOSHI TAIKI ,  TAKESHITA FUMIHIKO ,  KASUYA YUJI ,  NIWA TAKAKO ,  KOIZUMI MAKOTO

IPC分类号： C12N15/09 ,  A61K31/7125 ,  A61K31/713 ,  A61K39/02 ,  A61K39/12 ,  A61K39/39 ,  A61P31/04 ,  A61P31/12 ,  A61P33/00 ,  A61P35/00 ,  A61P37/04 ,  A61P37/08 ,  A61P43/00 ,  C07H21/04

CPC分类号： C12N15/117 ,  A61K31/7125 ,  A61K31/713 ,  A61K39/12 ,  A61K39/145 ,  A61K39/155 ,  A61K39/39 ,  A61K2039/5252 ,  A61K2039/55561 ,  A61K2039/55583 ,  C07H21/04 ,  C08B37/0024 ,  C12N7/00 ,  C12N2310/17 ,  C12N2310/315 ,  C12N2310/351 ,  C12N2310/3519 ,  C12N2320/31 ,  C12N2760/16134 ,  C12N2760/18534

摘要： The present invention provides an oligodeoxynucleotide containing humanized K type CpG oligodeoxynucleotide and poly deoxyadenylate, wherein the poly deoxyadenylate is placed on the 3'-side of the humanized K type CpG oligodeoxynucleotide. In addition, the present invention provides a complex containing the aforementioned oligodeoxynucleotide and ²-1,3-glucan.

摘要翻译： 本发明提供含有人源化K型CpG寡脱氧核苷酸和聚脱氧腺苷酸的寡聚脱氧核苷酸，其中聚脱氧腺苷酸置于人源化K型CpG寡脱氧核苷酸的3'侧。 另外，本发明提供了含有上述寡聚脱氧核苷酸和1,2-葡聚糖的复合物。

公开(公告)号：EP2985340A4

公开(公告)日：2016-11-02

申请号：EP14782456

申请日：2014-04-08

申请人： NAT INST OF BIOMEDICAL INNOVATION HEALTH AND NUTRITION ,  UNIV OSAKA

发明人： MIZUGUCHI HIROYUKI ,  KAWABATA KENJI ,  TAKAYAMA KAZUO ,  SEKIGUCHI KIYOTOSHI

IPC分类号： C12N5/071 ,  A61L27/38 ,  C12N5/0789 ,  C12N5/10 ,  C12N15/09

CPC分类号： C12N5/067 ,  A61L27/3834 ,  C12N2533/52

摘要： Provided is a method of stably maintaining and culturing "hepatoblast-like cells" generated during a differentiation-inducing process from pluripotent stem cells to hepatocytes. The present invention also provides a culture product obtained by the culture method. The hepatoblast-like cells can be maintained and cultured stably by bringing a laminin into contact with the hepatoblast-like cells. The method of the present invention which uses a laminin makes it possible for the first time to culture, maintain, and proliferate the hepatoblast-like cells. Desired mature cells such as mature hepatocytes and bile duct epithelial cells can be generated in a short time period and can be acquired at a desired timing by maintaining the hepatoblast-like cells. Further, the resulting cultured hepatoblast-like cells were demonstrated to be capable of being induced to differentiate into mature hepatocytes, mature cholangiocytes, bile duct epithelial cells, and the like. Thus, a substantially pure culture product of the present invention can be utilized as a composition for transplantation for use in regeneration of hepatocytes and/or bile duct epithelial cells.