公开(公告)号：EP1163372A4

公开(公告)日：2002-10-02

申请号：EP00919582

申请日：2000-03-23

申请人： PRINCETON SEPARATIONS

发明人： LEVISON DEREK

IPC分类号： C09B23/01 ,  C12Q1/68 ,  C07H21/00 ,  C09B23/00 ,  C12P19/34 ,  G01N33/533

CPC分类号： C09B23/0066

摘要： The invention describes novel plane-rigidized cyanine near-infrared (NIR) fluorescent dyes, their methods of preparation and use in the non-isotopic labeling of biological molecules. Such dyes contain a central cyclic hydroanthraquinone framework which provides for a stable and rigid structure to ensure low reactivity with neighboring molecules, a sharp absorption band, a high quantum yield, and emissions in a region largely devoid of natural background auto-fluorescence. Such dyes further comprise variable end groups flanking the central cyclic framework, allowing formation of a number of different plane-rigidized dyes, each having a slightly different emission wavelength. Functional groups are provided for attachment to target biological molecules. Moreover, hydrophilic functional groups are provided where necessary to significantly increase the water solubility of the dye molecules.

公开(公告)号：EP1153296A1

公开(公告)日：2001-11-14

申请号：EP00908658.8

申请日：2000-02-16

申请人： Princeton Separations

发明人： MOLLER, Uwe ,  LEVISON, Derek ,  LEVISON, Stuart

IPC分类号： G01N33/52 ,  G01N33/543

CPC分类号： G01N33/54393 ,  G01N33/582

摘要： The present invention relates to chemiluminescent assays which incorporate a second film or membrane which includes a solid chemical component for activation of a stable dioxetane. Decomposition of the stable dioxetane can be accomplished using a combination of heat and chemical treatment.

摘要翻译： 本发明涉及掺入第二膜或膜的化学发光测定法，所述第二膜或膜包含用于活化稳定的二氧杂环丁烷的固体化学成分。 稳定的二氧杂环丁烷的分解可以使用热和化学处理的组合来完成。

公开(公告)号：EP3811072A1

公开(公告)日：2021-04-28

申请号：EP19790334.7

申请日：2019-05-16

申请人： Princeton Separations Inc.

发明人： NIX, Paul, T. ,  HORN, Marcus, J.

IPC分类号： G01N27/447

公开(公告)号：EP1163372A1

公开(公告)日：2001-12-19

申请号：EP00919582.7

申请日：2000-03-23

申请人： Princeton Separations

发明人： LEVISON, Derek

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C09B23/0066

摘要： The invention describes novel plane-rigidized cyanine near-infrared (NIR) fluorescent dyes, their methods of preparation and use in the non-isotopic labeling of biological molecules. Such dyes contain a central cyclic hydroanthraquinone framework which provides for a stable and rigid structure to ensure low reactivity with neighboring molecules, a sharp absorption band, a high quantum yield, and emissions in a region largely devoid of natural background auto-fluorescence. Such dyes further comprise variable end groups flanking the central cyclic framework, allowing formation of a number of different plane-rigidized dyes, each having a slightly different emission wavelength. Functional groups are provided for attachment to target biological molecules. Moreover, hydrophilic functional groups are provided where necessary to significantly increase the water solubility of the dye molecules.

公开(公告)号：EP1153296A4

公开(公告)日：2002-07-17

申请号：EP00908658

申请日：2000-02-16

申请人： PRINCETON SEPARATIONS

发明人： MOLLER UWE ,  LEVISON DEREK ,  LEVISON STUART

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/52 ,  C12Q1/68

CPC分类号： G01N33/54393 ,  G01N33/582

摘要： The present invention relates to chemiluminescent assays which incorporate a second film or membrane which includes a solid chemical component for activation of a stable dioxetane. Decomposition of the stable dioxetane can be accomplished using a combination of heat and chemical treatment.

摘要翻译： 本发明涉及掺入第二膜或膜的化学发光测定法，所述第二膜或膜包含用于活化稳定的二氧杂环丁烷的固体化学成分。 稳定的二氧杂环丁烷的分解可以使用热和化学处理的组合来完成。