公开(公告)号：EP3071590A4

公开(公告)日：2017-07-19

申请号：EP14863946

申请日：2014-11-21

申请人： SENA RES INC

发明人： HUANG ZHEN

IPC分类号： A61K38/46 ,  C07K1/14 ,  C07K1/30 ,  C12N9/22 ,  C12N9/96 ,  G01N33/53 ,  G01N33/68 ,  G06F19/00

CPC分类号： A61K47/4823 ,  A61K38/465 ,  A61K47/61 ,  C07K1/30 ,  C12N9/22 ,  C12N9/96 ,  C12Y301/26004 ,  G01N33/5308 ,  G06F19/3437 ,  G16H50/50 ,  Y02A90/26

摘要： Methods for crystallizing a molecule of interest, such as a polypeptide, in complex with nucleic acid, including contacting the molecule of interest with selenium-derivatized nucleic acid and crystallizing the molecule of interest/selenium-derivatized nucleic acid complex are provided. Methods for determining the X-ray crystal structure of molecule of interest/selenium-derivatized nucleic acid complexes are also provided. Typically, the method of X-ray crystal structural determination includes selenium single-wavelength anomalous phasing of the selenium-derivatized nucleic acid. In some embodiments the phases for the X-ray crystal structure of the molecule of interest are not provided from another crystal. Also disclosed are methods of affecting a biological process by administering a functional nucleic acid to a cell or a subject and/or by bringing into contact a nuclease and a functional nucleic acid, where the functional nucleic acid is selenium-derivatized nucleic acid.

摘要翻译： 提供了使与核酸复合的感兴趣的分子例如多肽结晶的方法，包括使感兴趣的分子与硒衍生化的核酸接触并结晶目的分子/硒衍生的核酸复合物。 还提供了确定感兴趣的分子/硒衍生的核酸复合物的X射线晶体结构的方法。 通常，X射线晶体结构测定的方法包括硒衍生化的核酸的硒单波长异相定位。 在一些实施方案中，感兴趣分子的X射线晶体结构的相不是从另一种晶体提供的。 还公开了通过将功能性核酸给予细胞或受试者和/或通过使核酸酶和功能性核酸接触来影响生物过程的方法，其中功能性核酸是硒衍生的核酸。