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公开(公告)号：EP0777747A1
公开(公告)日：1997-06-11
申请号：EP95929169.0
申请日：1995-08-21
发明人： TULLY, Gillian , SULLIVAN, Kevin
IPC分类号： C12Q1
CPC分类号： C12Q1/6858 , C12Q1/6869
摘要： A method is provided for determining the identity of at least two discrete single nucleotide bases each adjacent to a predetermined nucleotide base sequence in a target sample comprising one or more types of polynucleotide chain, the method comprising incorporating a capture group into the target sample and immobilising the target sample by means of the capture group; mixing the target sample with (i) nucleotide primers which are complementary to the characterised base sequences such that they anneal thereto at positions adjacent to the bases to be identified and (ii) at least two types of dideoxynucleoside triphosphate (ddNTP), each type labelled with a distinguishable fluorescent group, and (iii) a nucleotide chain extending enzyme such that ddNTPs complementary to the bases to be identified are incorporated into the nucleotide primers; eluting the extending nucleotide primers, separating the types of extended nucleotide primer on basis of size or charge and identifying the ddNTPs incorporated into each type of nucleotide primer by reference to the fluorescent characteristics associated with the distinguishable groups. Primers and a test kit are provided for use in performance of the method.
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公开(公告)号：EP0135547B1
公开(公告)日：1988-08-03
申请号：EP84900869.3
申请日：1984-02-27
CPC分类号： C22C9/04 , A44C21/00 , Y10T428/12903
摘要： A coin, a coin blank or a metal strip for the production of a coin or a coin blank having as its surface a copper based alloy containing between 15 % and 30 % (by wt) zinc, between 2 % and 7 % (by wt) tin and, optionally, between 2 % and 7 % (by wt) nickel. The alloy is gold-coloured, tarnish resistant, fabricable and wear resistant.
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