公开(公告)号：EP0797774A1

公开(公告)日：1997-10-01

申请号：EP95940097.0

申请日：1995-12-14

申请人： 2860601 Canada Inc.

发明人： MALEEV, Alexander Michaelovich ,  BABLYUK, Yevgeney Borisovich ,  KOCHETOV, Ivan Alexandrovich ,  PARFENOV, Alexandr Sergeivich ,  LOPUKHIN, Yury Michaelovich ,  RABOVSKI, Alexandr B.

IPC分类号： G01N31 ,  C12Q1 ,  G01N33

CPC分类号： G01N33/92 ,  C12Q1/28 ,  C12Q1/60 ,  C12Q2326/00 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805

摘要： The invention relates to a multilayer analytical element for quantitatively measuring a component of a sample in the presence of a reagent capable of interacting with the sample. The multilayer analytical element of the invention comprises a solid support and a hydrophilic layer thereon, the hydrophilic layer being made of gelatin, polyvinyl alcohol or a mixture thereof and containing a developing agent and a color-producing agent in a total amount of 5 to 30 weight %, the developing agent and color-producing agent being present in a weight ratio of 1:0.3 to 1:3.0. The developing agent is capable of undergoing oxidation caused by the reagent in the presence of an oxidizing agent, whereby the developing agent in oxidized form reacts with the color-producing agent to form a colored product. The multilayer analytical element according to the invention can be used in medicine and pharmacology as well as in criminal law. It can be utilized for measuring cholesterol, chloride ions and glucose, thereby enabling the diagnosis of hypercholesterolemia, cysticfibrosis and diabetes.