公开(公告)号：EP0460576B1

公开(公告)日：1996-08-28

申请号：EP91109036.3

申请日：1991-06-03

申请人： ABBOTT LABORATORIES

发明人： Garner, William D. ,  Codacovi, Lynn Marie ,  Mattingly, Phillip Gregory ,  Welch, Christopher Joseph ,  Kuemmerle, Steven C. ,  Delby, Suzanne M. ,  Lane, Theresa L.

IPC分类号： G01N33/82 ,  G01N33/543 ,  G01N33/546 ,  G01N33/545

CPC分类号： G01N33/54353 ,  G01N33/82

公开(公告)号：EP0460576A1

公开(公告)日：1991-12-11

申请号：EP91109036.3

申请日：1991-06-03

申请人： ABBOTT LABORATORIES

发明人： Garner, William D. ,  Codacovi, Lynn Marie ,  Mattingly, Phillip Gregory ,  Welch, Christopher Joseph ,  Kuemmerle, Steven C. ,  Delby, Suzanne M. ,  Lane, Theresa L.

IPC分类号： G01N33/82 ,  G01N33/543 ,  G01N33/546 ,  G01N33/545

CPC分类号： G01N33/54353 ,  G01N33/82

摘要： The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

摘要翻译： 本发明是用于检测样品中钴胺素的方法，试剂盒和试剂。 该方法包括向样品中引入与亲和纯化的内在因子连接的胶乳或胶乳样固相的第一缀合物与至少三埃长的第一连接基团。 然后将样品中的第一缀合物和结合的钴胺素暴露于与可检测酶连接的钴胺素的第二偶联物，以产生与第一偶联物和未结合的第二偶联物结合的第二缀合物。 然后检测与固相第一偶联物或未结合的第二偶联物相关的酶活性。 通过将亲和纯化的固有因子与至少三埃长的第一连接基团连接到胶乳或胶乳样固相上，可以进行酶测定，其检测在这些样品中发现的正常范围内或低于正常范围内或低于正常范围内的患者样品中的钴胺素的水平。