公开(公告)号：EP2333049A1

公开(公告)日：2011-06-15

申请号：EP10179746.2

申请日：2000-09-25

申请人： ABT Holding Company

发明人： Young, Henry E. ,  Lucas, Paul A.

IPC分类号： C12N5/10 ,  G01N33/50 ,  A61K35/12 ,  A61K48/00

CPC分类号： G01N33/5073 ,  C12N5/0607 ,  C12N5/0663 ,  C12N5/0668

摘要： The present invention relates to pluripotent stem cells, particularly to pluripotent embryonic-like stem cells. The invention further relates to methods of purifying pluripotent embryonic-like stem cells and to compositions, cultures and clones thereof. The present invention also relates to a method of transplanting the pluripotent stem cells of the present invention in a mammalian host, such as human, comprising introducing the stem cells, into the host. The invention further relates to methods of in vivo administration of a protein or gene of interest comprising transfecting a pluripotent stem cell with a construct comprising DNA which encodes a protein of interest and then introducing the stem cell into the host where the protein or gene of interest is expressed. The present invention also relates to methods of producing mesodermal, endodermal or ectodermal lineage-committed cells by culturing or transplantation of the pluripotent stem cells of the present invention.

摘要翻译： 本发明涉及多能干细胞，特别涉及多能胚胎样干细胞。 本发明还涉及纯化多能胚胎样干细胞及其组合物，培养物和克隆的方法。 本发明还涉及将本发明的多能干细胞移植到哺乳动物宿主例如人中的方法，包括将干细胞导入宿主。 本发明还涉及体内施用目的蛋白质或基因的方法，包括用包含编码目的蛋白质的DNA的构建体转染多能干细胞，然后将干细胞导入宿主中，其中感兴趣的蛋白质或基因 被表达。 本发明还涉及通过培养或移植本发明的多能干细胞来生产中胚层，内胚层或外胚层谱系定型细胞的方法。