专利检索 ap:("AFFYMAX TECHNOLOGIES N.V.") AND inv:"CRAMERI, Andreas" 第 1 页

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发明公开
DNA MUTAGENESIS BY RANDOM FRAGMENTATION AND REASSEMBLY 失效
标题翻译： DNA突变偶然的裂变与重构

公开(公告)号：EP0752008A1

公开(公告)日：1997-01-08

申请号：EP95911826.0

申请日：1995-02-17

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： STEMMER, Willem P.C. , CRAMERI, Andreas

IPC分类号： C12N15 , A61K38 , A61K39 , C07K14 , C07K16 , C12N9 , C12P21 , C12Q1 , C40B40 , G01N33

CPC分类号： C40B40/02 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C12Y305/02006 , C40B40/08 , C40B50/06

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.